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SEMA6D ELISA Kit

SEMA6D Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4884465
  • Target Alle SEMA6D Produkte
    SEMA6D (Sema Domain, Transmembrane Domain (TM), and Cytoplasmic Domain, (Semaphorin) 6D (SEMA6D))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human Semaphorin 6D ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Human Semaphorin 6D.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle SEMA6D Produkte
    SEMA6D (Sema Domain, Transmembrane Domain (TM), and Cytoplasmic Domain, (Semaphorin) 6D (SEMA6D))
    Andere Bezeichnung
    Semaphorin 6D (SEMA6D Produkte)
    Synonyme
    1110067B02Rik ELISA Kit, AA409156 ELISA Kit, D330011G23 ELISA Kit, Sema6D-1 ELISA Kit, Sema6D-2 ELISA Kit, Sema6D-4 ELISA Kit, Sema6D-5 ELISA Kit, Sema6D-6 ELISA Kit, mKIAA1479 ELISA Kit, wu:fi06a12 ELISA Kit, wu:fi76a10 ELISA Kit, zgc:56170 ELISA Kit, semaphorin 6D ELISA Kit, sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D ELISA Kit, semaphorin 6D L homeolog ELISA Kit, SEMA6D ELISA Kit, Sema6d ELISA Kit, sema6d ELISA Kit, sema6d.L ELISA Kit
    Gen-ID
    80031
    UniProt
    Q8NFY4
    Pathways
    Smooth Muscle Cell Migration
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