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SEMA6D ELISA Kit
SEMA6D
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4884465
Produktdetails SEMA6D ELISA Kit
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Target
Alle SEMA6D Produkte
SEMA6D
(Sema Domain, Transmembrane Domain (TM), and Cytoplasmic Domain, (Semaphorin) 6D (SEMA6D))
Reaktivität
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Human Semaphorin 6D ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Human Semaphorin 6D.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternativen
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu SEMA6D
(ausblenden)
Target
Alle SEMA6D Produkte
SEMA6D
(Sema Domain, Transmembrane Domain (TM), and Cytoplasmic Domain, (Semaphorin) 6D (SEMA6D))
Andere Bezeichnung
Semaphorin 6D (SEMA6D Produkte )
Synonyme
1110067B02Rik ELISA Kit, AA409156 ELISA Kit, D330011G23 ELISA Kit, Sema6D-1 ELISA Kit, Sema6D-2 ELISA Kit, Sema6D-4 ELISA Kit, Sema6D-5 ELISA Kit, Sema6D-6 ELISA Kit, mKIAA1479 ELISA Kit, wu:fi06a12 ELISA Kit, wu:fi76a10 ELISA Kit, zgc:56170 ELISA Kit, semaphorin 6D ELISA Kit, sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D ELISA Kit, semaphorin 6D L homeolog ELISA Kit, SEMA6D ELISA Kit, Sema6d ELISA Kit, sema6d ELISA Kit, sema6d.L ELISA Kit
Gen-ID
80031
UniProt
Q8NFY4
Pathways
Smooth Muscle Cell Migration
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