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RALB ELISA Kit
Ralb
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4884317
Produktdetails RALB ELISA Kit
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Target
Alle RALB (Ralb) ELISA Kits anzeigen
RALB (Ralb)
(V-Ral Simian Leukemia Viral Oncogene Homolog B (Ras Related, GTP Binding Protein) (Ralb))
Reaktivität
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human RalB ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes .
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu RALB
(ausblenden)
Target
Alle RALB (Ralb) ELISA Kits anzeigen
RALB (Ralb)
(V-Ral Simian Leukemia Viral Oncogene Homolog B (Ras Related, GTP Binding Protein) (Ralb))
Andere Bezeichnung
RalB (Ralb Produkte )
Synonyme
dRalb ELISA Kit, 5730472O18Rik ELISA Kit, Ral B ELISA Kit, ralb ELISA Kit, xralb ELISA Kit, zgc:100801 ELISA Kit, KRAS2 ELISA Kit, k-ras ELISA Kit, RAS like proto-oncogene B ELISA Kit, v-ral simian leukemia viral oncogene B ELISA Kit, v-ral simian leukemia viral oncogene homolog B L homeolog ELISA Kit, v-ral simian leukemia viral oncogene homolog Ba (ras related) ELISA Kit, v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein) ELISA Kit, RALB ELISA Kit, Ralb ELISA Kit, ralb.L ELISA Kit, ralba ELISA Kit, ralb ELISA Kit
Gen-ID
5899
UniProt
P11234
Pathways
Neurotrophin Signalübertragung , CXCR4-mediated Signaling Events
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