Plexin A4 ELISA Kit

Produktnummer ABIN4884198

Produktdetails Plexin A4 ELISA Kit

Target: Plexin A4 (PLXNA4)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human Plexin A4 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Plexin A4

Target: Plexin A4 (PLXNA4)

Andere Bezeichnung: Plexin A4 (PLXNA4 Produkte)

Synonyme: D204 ELISA Kit, wu:fd49b01 ELISA Kit, wu:fe15f03 ELISA Kit, PLXNA4A ELISA Kit, FAYV2820 ELISA Kit, PLEXA4 ELISA Kit, PLXNA4B ELISA Kit, PRO34003 ELISA Kit, 9330117B14 ELISA Kit, Plxa4 ELISA Kit, mKIAA1550 ELISA Kit, PLEX2 ELISA Kit, Plxna4 ELISA Kit, PLXNA4 ELISA Kit, plexin A4 ELISA Kit, plexin-A4 ELISA Kit, plxna4 ELISA Kit, PLXNA4 ELISA Kit, LOC100467706 ELISA Kit, Plxna4 ELISA Kit

Gen-ID: 91584

UniProt: Q9HCM2

Pathways: Regulation of Cell Size