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TNFRSF4 ELISA Kit

TNFRSF4 Reaktivität: Human Colorimetric Sandwich ELISA 3.28-800 pg/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4884060
  • Target Alle TNFRSF4 ELISA Kits anzeigen
    TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
    Reaktivität
    • 5
    • 4
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    3.28-800 pg/mL
    Untere Nachweisgrenze
    3.28 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Human OX40/TNFRSF4 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Human OX40.
    Sensitivität
    3.2 pg/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle TNFRSF4 ELISA Kits anzeigen
    TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
    Andere Bezeichnung
    OX40 (TNFRSF4 Produkte)
    Synonyme
    TNFRSF4 ELISA Kit, ACT35 ELISA Kit, CD134 ELISA Kit, Ly-70 ELISA Kit, Ox40 ELISA Kit, TXGP1L ELISA Kit, Txgp1 ELISA Kit, OX40 ELISA Kit, TNF receptor superfamily member 4 ELISA Kit, tumor necrosis factor receptor superfamily, member 4 ELISA Kit, TNFRSF4 ELISA Kit, Tnfrsf4 ELISA Kit
    Gen-ID
    7293
    UniProt
    P43489
    Pathways
    Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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