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KIR2DL3 ELISA Kit
KIR2DL3
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4883506
Produktdetails KIR2DL3 ELISA Kit
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Target
Alle KIR2DL3 ELISA Kits anzeigen
KIR2DL3
(Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 3 (KIR2DL3))
Reaktivität
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human KIR2DL3/CD158b2 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes .
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu KIR2DL3
(ausblenden)
Target
Alle KIR2DL3 ELISA Kits anzeigen
KIR2DL3
(Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 3 (KIR2DL3))
Andere Bezeichnung
KIR2DL3 (KIR2DL3 Produkte )
Synonyme
CD158B2 ELISA Kit, CD158b ELISA Kit, GL183 ELISA Kit, KIR-023GB ELISA Kit, KIR-K7b ELISA Kit, KIR-K7c ELISA Kit, KIRCL23 ELISA Kit, NKAT ELISA Kit, NKAT2 ELISA Kit, NKAT2A ELISA Kit, NKAT2B ELISA Kit, p58 ELISA Kit, killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 3 ELISA Kit, KIR2DL3 ELISA Kit
Gen-ID
3804
UniProt
P43628
Pathways
Cancer Immune Checkpoints
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