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CSF2RA ELISA Kit
CSF2RA
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4883100
Produktdetails CSF2RA ELISA Kit
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Target
Alle CSF2RA ELISA Kits anzeigen
CSF2RA
(Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA))
Reaktivität
Alle Reaktivitäten für CSF2RA ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Human GM-CSF R alpha ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Human GM-CSF R alpha.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu CSF2RA
(ausblenden)
Target
Alle CSF2RA ELISA Kits anzeigen
CSF2RA
(Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA))
Andere Bezeichnung
GM-CSF R alpha (CSF2RA Produkte )
Synonyme
CD116 ELISA Kit, CDw116 ELISA Kit, CSF2R ELISA Kit, CSF2RAX ELISA Kit, CSF2RAY ELISA Kit, CSF2RX ELISA Kit, CSF2RY ELISA Kit, GM-CSF-R-alpha ELISA Kit, GMCSFR ELISA Kit, GMR ELISA Kit, SMDP4 ELISA Kit, Csfgmra ELISA Kit, GM-CSF-Ra ELISA Kit, GM-CSFR ELISA Kit, colony stimulating factor 2 receptor, alpha ELISA Kit, colony stimulating factor 2 receptor alpha subunit ELISA Kit, colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) ELISA Kit, CSF2RA ELISA Kit, Csf2ra ELISA Kit
Gen-ID
1438
UniProt
P15509
Pathways
JAK-STAT Signalweg
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