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FGF20 ELISA Kit

FGF20 Reaktivität: Human Colorimetric Sandwich ELISA 0.3-75 ng/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4882860
  • Target Alle FGF20 ELISA Kits anzeigen
    FGF20 (Fibroblast Growth Factor 20 (FGF20))
    Reaktivität
    • 2
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.3-75 ng/mL
    Untere Nachweisgrenze
    0.3 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Human FGF-20 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Proben
    Cell Culture Supernatant, Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes human FGF-20. Other species not yet determined.
    Sensitivität
    0.3 ng/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle FGF20 ELISA Kits anzeigen
    FGF20 (Fibroblast Growth Factor 20 (FGF20))
    Andere Bezeichnung
    FGF-20 (FGF20 Produkte)
    Synonyme
    FGF-20 ELISA Kit, fgf20 ELISA Kit, fgf20.S ELISA Kit, xfgf-20 ELISA Kit, xfgf20 ELISA Kit, dob ELISA Kit, wu:fb05c08 ELISA Kit, fibroblast growth factor 20 ELISA Kit, fibroblast growth factor 20 L homeolog ELISA Kit, fibroblast growth factor 20a ELISA Kit, FGF20 ELISA Kit, Fgf20 ELISA Kit, fgf20.L ELISA Kit, fgf20a ELISA Kit
    Hintergrund
    Gene Names: FGF20
    Protein names: Fibroblast growth factor 20 (FGF-20)
    Gen-ID
    26281
    UniProt
    Q9NP95
    Pathways
    Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung
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