DSCAM ELISA Kit

Produktnummer ABIN4882662

Produktdetails DSCAM ELISA Kit

Target: DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human DSCAM ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu DSCAM

Target: DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))

Andere Bezeichnung: DSCAM (DSCAM Produkte)

Synonyme: CHD2-42 ELISA Kit, CHD2-52 ELISA Kit, Dscam ELISA Kit, DSCAM ELISA Kit, chd2-42 ELISA Kit, chd2-52 ELISA Kit, dscam ELISA Kit, GB15141 ELISA Kit, 4932410A21Rik ELISA Kit, 43Bc ELISA Kit, CG17800 ELISA Kit, CT39257 ELISA Kit, DScam ELISA Kit, DmDscam ELISA Kit, Dm_2R:13612 ELISA Kit, Dmel\\CG17800 ELISA Kit, Dscam-hv ELISA Kit, Dscam1 ELISA Kit, FBgn0033159 ELISA Kit, Neu1 ELISA Kit, dScam ELISA Kit, l(2)05518 ELISA Kit, l(2)43Bc ELISA Kit, p270 ELISA Kit, DS cell adhesion molecule ELISA Kit, Down syndrome cell adhesion molecule ELISA Kit, Down syndrome cell adhesion molecule a ELISA Kit, Down syndrome cell adhesion molecule 1 ELISA Kit, Down syndrome cell adhesion molecule-like protein Dscam2 ELISA Kit, down syndrome cell adhesion molecule ELISA Kit, Down syndrome cell adhesion molecule L homeolog ELISA Kit, DSCAM ELISA Kit, Dscam ELISA Kit, dscam ELISA Kit, dscama ELISA Kit, Dscam1 ELISA Kit, LOC5573626 ELISA Kit, CpipJ_CPIJ006173 ELISA Kit, dscam.L ELISA Kit

Gen-ID: 1826

UniProt: O60469

Pathways: Regulation of Cell Size