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KLRK1 ELISA Kit
KLRK1
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4882179
Produktdetails KLRK1 ELISA Kit
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Target
Alle KLRK1 ELISA Kits anzeigen
KLRK1
(Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))
Reaktivität
Alle Reaktivitäten für KLRK1 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human CD314 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Human GATA-3.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternativen
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu KLRK1
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Target
Alle KLRK1 ELISA Kits anzeigen
KLRK1
(Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))
Andere Bezeichnung
CD314 (KLRK1 Produkte )
Synonyme
KLRK1 ELISA Kit, NKG2D ELISA Kit, CD314 ELISA Kit, D12S2489E ELISA Kit, KLR ELISA Kit, NKG2-D ELISA Kit, D6H12S2489E ELISA Kit, Nkg2d ELISA Kit, Nkrp2 ELISA Kit, killer cell lectin like receptor K1 ELISA Kit, killer cell lectin-like receptor subfamily K, member 1 ELISA Kit, NKG2D protein ELISA Kit, KLRK1 ELISA Kit, Klrk1 ELISA Kit, NKG2D ELISA Kit
Gen-ID
100528032, 22914
UniProt
P26718
Pathways
Activation of Innate immune Response , Cellular Response to Molecule of Bacterial Origin , Regulation of Leukocyte Mediated Immunity , Positive Regulation of Immune Effector Process
Zuletzt angesehen
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