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ADAMTS13 ELISA Kit
ADAMTS13
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4881751
Produktdetails ADAMTS13 ELISA Kit
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Target
Alle ADAMTS13 ELISA Kits anzeigen
ADAMTS13
(ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13))
Reaktivität
Alle Reaktivitäten für ADAMTS13 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human ADAMTS-13 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
The antibody pair provided in this kit recognizes Human ADAMTS-13
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu ADAMTS13
(ausblenden)
Target
Alle ADAMTS13 ELISA Kits anzeigen
ADAMTS13
(ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13))
Andere Bezeichnung
ADAMTS-13 (ADAMTS13 Produkte )
Synonyme
ADAMTS13 ELISA Kit, ADAM-TS13 ELISA Kit, ADAMTS-13 ELISA Kit, C9orf8 ELISA Kit, VWFCP ELISA Kit, vWF-CP ELISA Kit, Gm710 ELISA Kit, ADAM metallopeptidase with thrombospondin type 1 motif 13 ELISA Kit, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 13 ELISA Kit, ADAM metallopeptidase with thrombospondin type 1 motif, 13 ELISA Kit, ADAMTS13 ELISA Kit, Adamts13 ELISA Kit, adamts13 ELISA Kit
Gen-ID
11093
UniProt
Q76LX8
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