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TNFRSF12A ELISA Kit

TNFRSF12A Reaktivität: Pferd Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4881621
  • Target Alle TNFRSF12A ELISA Kits anzeigen
    TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))
    Reaktivität
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    Pferd
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Equine TWEAK Receptor (TNFRSF12A) ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes Equine TWEAK Receptor (TNFRSF12A)
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product TNFRSF12A ELISA Kit
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle TNFRSF12A ELISA Kits anzeigen
    TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))
    Andere Bezeichnung
    TWEAK R (TNFRSF12A Produkte)
    Synonyme
    CD266 ELISA Kit, FN14 ELISA Kit, TWEAKR ELISA Kit, AI255180 ELISA Kit, C87282 ELISA Kit, Fn14 ELISA Kit, HPIP ELISA Kit, TWEAK-R ELISA Kit, TweakR ELISA Kit, TNF receptor superfamily member 12A ELISA Kit, tumor necrosis factor receptor superfamily, member 12a ELISA Kit, TNFRSF12A ELISA Kit, Tnfrsf12a ELISA Kit
    Pathways
    Apoptose, Regulation of Cell Size
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