Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

Urocortin ELISA Kit

UCN Reaktivität: Maus, Human, Ratte Colorimetric Competition ELISA 0.1-1.000 ng/mL Serum
Produktnummer ABIN4881378
  • Target Alle Urocortin (UCN) ELISA Kits anzeigen
    Urocortin (UCN)
    Reaktivität
    Maus, Human, Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Competition ELISA
    Detektionsbereich
    0.1-1.000 ng/mL
    Untere Nachweisgrenze
    0.1 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Human/Mouse/Rat Urocortin EIA Kit optimized for serum. Competition-based ELISA on a 96-well strip plate.
    Proben
    Serum
    Analytische Methode
    Quantitative
    Spezifität
    This EIA kit is designed to detect human, mouse, and rat active Urocortin.
    Sensitivität
    1.2 ng/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Standard Peptide
    • Assay Diluent(s)
    • Biotinylated Peptide
    • HRP-Streptavidin
    • TMB One-Step Substrate
    • Stop Solution
    • Assay Diagram
    • Positive Control Sample
    • Capture Antibody
    • User Manual
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Orbital shaker
    • Aluminum foil
    • Saran Wrap
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
    Top Product
    Discover our top product UCN ELISA Kit
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 μL detection antibody to each well.
    3. Incubate 1.5 h at RT or O/N at 4 °C.
    4. Add 100 μL standard or sample to each well.
    5. Incubate 2.5 h at RT.
    6. Add 100 μL prepared streptavidin solution.
    7. Incubate 45 min at RT.
    8. Add 100 μL TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL Stop Solution to each well.
    11. Read plate at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Standard, Biotinylated Urocortin peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
    Haltbarkeit
    6 months
  • Target Alle Urocortin (UCN) ELISA Kits anzeigen
    Urocortin (UCN)
    Andere Bezeichnung
    Urocortin (UCN Produkte)
    Synonyme
    UCN ELISA Kit, si:ch211-243j20.4 ELISA Kit, ucn ELISA Kit, wu:fq17a01 ELISA Kit, UI ELISA Kit, UROC ELISA Kit, Mpv17 ELISA Kit, Ucn1 ELISA Kit, urocortin ELISA Kit, urotensin 1 ELISA Kit, UCN ELISA Kit, uts1 ELISA Kit, Ucn ELISA Kit
    Gen-ID
    22226
    UniProt
    P81615
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of Cell Size, Feeding Behaviour
Sie sind hier:
Kundenservice