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ADIPOQ ELISA Kit

ADIPOQ Reaktivität: Maus Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN415581
  • Target Alle ADIPOQ ELISA Kits anzeigen
    ADIPOQ (Adiponectin (ADIPOQ))
    Reaktivität
    • 10
    • 7
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    31.2 pg/mL - 2000 pg/mL
    Untere Nachweisgrenze
    31.2 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ADP in Serum,Plasma,Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität

    This assay has high sensitivity and excellent specificity for detection of Adiponectin (ADP).
    No significant cross-reactivity or interference between Adiponectin (ADP) and analogues was observed.

    Kreuzreaktivität (Details)
    No significant cross-reactivity or interference between Adiponectin (ADP) and analogues was observed.
    Sensitivität
    14.2 pg/mL
    Bestandteile
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (30 x concentrate)
    • Instruction manual
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  • Applikationshinweise
    • Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
    • The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
    • Kits from different batches may be a little different in detection range, sensitivity and color developing time.
    • Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
    • Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
    • There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
    • Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
    • Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
    • Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
    • Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.
    Kommentare

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    Probenmenge
    50 μL
    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adiponectin (ADP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adiponectin (ADP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adiponectin (ADP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adiponectin (ADP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Aufbereitung der Reagenzien
    1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
    2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 20ng/mL. Firstly dilute the stock solution to 10ng/mL and the diluted standard serves as the highest standard (10ng/mL). Then prepare 7 tubes containing 0.5 mL Standard Diluent and use the diluted standard to produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312g/mL, 0.156ng/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0pg/mL.
    3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
    4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
    5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

    Note:

    1. Making serial dilution in the wells directly is not permitted.
    2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
    3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
    4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
    6. Contaminated water or container for reagent preparation will influence the detection result.
    Testpräzision

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adiponectin (ADP) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adiponectin (ADP) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Vorsichtsmaßnahmen
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Handhabung
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
    • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
      Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
    • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
    Haltbarkeit
    6 months
  • Wolf, Bukosza, Engel, Poggi, Jehle, Anto Michel, Chen, Colberg, Hoppe, Dufner, Boon, Blankenbach, Hilgendorf, von Zur Muhlen, Reinöhl, Sommer, Marchini, Febbraio, Weber, Bode, Peter, Lutgens, Zirlik: "Inflammation, but not recruitment, of adipose tissue macrophages requires signalling through Mac-1 (CD11b/CD18) in diet-induced obesity (DIO)." in: Thrombosis and haemostasis, Vol. 117, Issue 2, pp. 325-338, (2018) (PubMed).

    Chi, Li, Li, Chen, Ma, Peng, Pan, Li, Yu, He, Geng, Cui, Liu, Yang: "FAM3A enhances adipogenesis of 3T3-L1 preadipocytes via activation of ATP-P2 receptor-Akt signaling pathway." in: Oncotarget, Vol. 8, Issue 28, pp. 45862-45873, (2018) (PubMed).

    Lu, Chen, Xian, Zhou, Yuan, Wang, Gan, Wang, Xiong, Ma, Yu, Huang: "Epigallocatechin-3-gallate suppresses differentiation of adipocytes via regulating the phosphorylation of FOXO1 mediated by PI3K-AKT signaling in 3T3-L1 cells." in: Oncotarget, Vol. 9, Issue 7, pp. 7411-7423, (2018) (PubMed).

    Han, Li, Pan, Xu, Han, Hou, Sun: "Calycosin directly improves perivascular adipose tissue dysfunction by upregulating the adiponectin/AMPK/eNOS pathway in obese mice." in: Food & function, Vol. 9, Issue 4, pp. 2409-2415, (2018) (PubMed).

    Pang, Peng, Zhang, Wang, Jia, Bi, Chen: "Bushenkangshuai Tablet Reduces Atherosclerotic Lesion by Improving Blood Lipids Metabolism and Inhibiting Inflammatory Response via TLR4 and NF-κB Signaling Pathway." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 1758383, (2018) (PubMed).

    Hou, Liu, Han, Wang, Hou, Hou, Sun: "Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese mice." in: Journal of molecular and cellular cardiology, Vol. 99, pp. 188-196, (2016) (PubMed).

    Li, Jiang, Yang, Wu, Sun, Sun: "CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes." in: Endocrine journal, Vol. 61, Issue 12, pp. 1153-62, (2015) (PubMed).

    Yang, Zhang, Zhen, Gao, Du, Xiao, Wang, Ge, Hu, Ye, Zhu, Li: "Impaired adipogenesis in adipose tissue associated with hepatic lipid deposition induced by chronic inflammation in mice with chew diet." in: Life sciences, Vol. 137, pp. 7-13, (2015) (PubMed).

    Nie, Yan, Yan, Xia, Zhang: "Cold exposure stimulates lipid metabolism, induces inflammatory response in the adipose tissue of mice and promotes the osteogenic differentiation of BMMSCs via the p38 MAPK pathway in vitro." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10875-86, (2015) (PubMed).

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    Wolf, Jehle, Ortiz Rodriguez, Dufner, Hoppe, Colberg, Lozhkin, Bassler, Rupprecht, Wiedemann, Hilgendorf, Stachon, Willecke, Febbraio, von zur Muhlen, Binder, Bode, Zirlik, Peter: "CD40L deficiency attenuates diet-induced adipose tissue inflammation by impairing immune cell accumulation and production of pathogenic IgG-antibodies." in: PLoS ONE, Vol. 7, Issue 3, pp. e33026, (2012) (PubMed).

    Goodman et al.: "The role of arachidonic acid metabolites in cardiovascular homeostasis. Biochemical, histological and clinical cardiovascular effects of non-steroidal anti-inflammatory drugs and their interactions ..." in: Drugs, Vol. 33 Suppl 1, pp. 47-55, (1987) (PubMed).

  • Target Alle ADIPOQ ELISA Kits anzeigen
    ADIPOQ (Adiponectin (ADIPOQ))
    Andere Bezeichnung
    Adiponectin (ADPN) (ADIPOQ Produkte)
    Synonyme
    ADP ELISA Kit, DCAF9 ELISA Kit, 6720489L24Rik ELISA Kit, 9330209L04Rik ELISA Kit, Adp ELISA Kit, Mamdc1 ELISA Kit, RNSVSP4U ELISA Kit, SVSIV1 ELISA Kit, Svp4 ELISA Kit, ACDC ELISA Kit, ACRP30 ELISA Kit, ADIPQTL1 ELISA Kit, ADPN ELISA Kit, APM-1 ELISA Kit, APM1 ELISA Kit, GBP28 ELISA Kit, apM-1 ELISA Kit, 30kDa ELISA Kit, APN ELISA Kit, Acdc ELISA Kit, Acrp30 ELISA Kit, Ad ELISA Kit, adipo ELISA Kit, apM1 ELISA Kit, ADN ELISA Kit, acrpl ELISA Kit, adipo-b ELISA Kit, adipoql ELISA Kit, zgc:153584 ELISA Kit, adipoq ELISA Kit, WD and tetratricopeptide repeats 1 ELISA Kit, MAM domain containing glycosylphosphatidylinositol anchor 2 ELISA Kit, seminal vesicle secretory protein 4 ELISA Kit, adiponectin, C1Q and collagen domain containing ELISA Kit, adiponectin, C1Q and collagen domain containing, b ELISA Kit, adiponectin, C1Q and collagen domain containing L homeolog ELISA Kit, WDTC1 ELISA Kit, Mdga2 ELISA Kit, Svs4 ELISA Kit, ADIPOQ ELISA Kit, Adipoq ELISA Kit, adipoqb ELISA Kit, adipoq.L ELISA Kit
    UniProt
    Q60994
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