Adiponectin (mouse) Elisa Assay Kit

Details zu Produkt Nr. ABIN411775, Anbieter: Anmelden zum Anzeigen
  • ACDC
  • ACRP30
  • ADPN
  • APM-1
  • APM1
  • GBP28
  • apM-1
  • 30kDa
  • APN
  • Acdc
  • Acrp30
  • Ad
  • adipo
  • apM1
  • ADN
  • adipoq
  • adiponectin, C1Q and collagen domain containing
  • adiponectin, C1Q and collagen domain containing L homeolog
  • Adipoq
  • adipoq.L
Biochemical Assay (BCA)
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Hersteller Produkt- Nr.
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Proben Cell Culture Supernatant, Plasma, Serum, Tissue Samples
Nachweismethode Colorimetric
Produktmerkmale Mouse Adiponectin ELISA Assay Kit: Sensitive & Convenient Assay for Quantitative Determination of Adiponectin in Mouse Serum, Plasma or Various Tissue or Cell Culture supernatants.
Plate is coated with a monoclonal antibody specific for adiponectin. Detection antibody is a polyclonal antibody specific for adiponectin.
Bestandteile Adiponectin antibody-coated plate, 6x16 wells Wash concentrate (10X), 2 x 30 ml Diluent (10X), 2 x 30 ml Secondary Antibody (200X), 60 μl Detector (100X), 150 μl Mouse adiponectin Standard (lyophilized), 16 ng TMB, 12 ml Stop Solution, 12 ml Plate sealers, 2
Andere Bezeichnung Adiponectin (ADIPOQ ELISA Kit Abstract)
Hintergrund Adipose tissue secretes a number of biologically active soluble factors (collectively named adipocytokines) that regulate glucose and fatty acid metabolism. Measurement of serum adiponectin levels gives us important information on the role of adiponectin in regulation of glucose and/or lipid metabolism. This Mouse Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in mouse serum, plasma or various tissue or cell culture supernatants. In the assay, monoclonal antibody specific for mouse adiponectin has been pre-coated onto 96 well microplate. Standards and samples are pipetted into the wells and adiponectin present is bound by immobilized antibody. The bound adiponectin is then captured by anti-mouse adiponectin polyclonal antibody. With HRP conjugated anti-rabbit IgG and a HRP substrate, the colors developed in proportion to the bound adiponectin, can be easily measured by Elisa plate reader.
Applikationshinweise This mouse Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in mouse serum, plasma or various tissue or cell culture supernatants

Further details regarding sample type: Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids

Protokoll A. Preparation of Reagents 1. Allow all samples and kit components to equilibrate to room temperature (20-25 °C).
2. Plan the plate configuration and create a plate map. Calculate the amount of working reagents to use (See table below). It is recommended that standards and samples be run in duplicate.
3. Prepare 1X Wash Solution: Dilute 5X Wash Concentrate to 1X with deionized water. The diluted 1X Wash Solution is stable for one month at room temprature
4. Prepare 1X Diluent. Dilute 5X Diluent to 1X with deionized water.
5. Prepare 1X Detector. Dilute 100X Detector to 1X wth 1X Diluent. Use the 1X Detector within one hour of preparation.
6. Prepare Substrate Solution freshly by adding one part Substrate I to one part Substrate II. Freshly prepare just before use. The amount of working reagents needed for 1 well Working reagents Total volume needed Stock solution added Dilution solution added Note 1X Wash Solution 2.8 mL 0.56 mL of 5X Wash Concentrate 2.24 mL of ddH2O Stable for 1 month at RT 1X Diluent 2.5 mL 0.5 mL of 5X Diluent 2 mL of ddH2O in the case of 10 µL sample, Including standard dilution 1X Detector 110 µL 1.1 µL of 100X Detector 108.9 µL of 1X Diluent Use within 1 hr. Substrate Solution 110 µL 55 µL of Substrate I 55 µL of Substrate II Freshly repared just before use
7. Prepare working aliquots of the Standard as follows: Briefly centrifuge the lyophilzed Standard vial. When opening, remove cap gently as the lyophilizate may have become dislodged during shipping. Add 1 mL of deionized water the Standard vial to make a stock concentration of 48 ng/mL. Mix well. A recommended dilution scheme is as follows : 1) Label 8 microcentrifuge tubes #0-7. Add 125 µL Diluent to the microcentrifuge tubes #0-7. 2) Add 125 µL of the stock Standard solution to tube #7 and vortex. This is Standard tube #7 with a concentration of 24 ng/mL 3) Standards #6 to #1 are then prepared by performing a 1:2 dilution of the preceding standard. Do not add any standard to the tube #0 125 µL 125 µL 125 µL 125 µL 125 µL 125 µL 125 µL stock Standard Tube # stock 7 6 5 4 3 2 1 0 Conc.(ng/mL) 16 8 4 2 1 0.5 0.25 0.125 0 Initial vol.(µL) - 125 125 125 125 125 125 125 250
8. Reconstitute QC sample in 1 mL of deionized water. The Kit 01/06
Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung 4 °C
Haltbarkeit 12 months
Bilder des Herstellers
ELISA image for Adiponectin (mouse) Elisa Assay Kit (ABIN411775) Adiponectin (mouse) Elisa Assay Kit
Produkt verwendet in: Solberg, Robertson, Aronsen, Rognmo, Sjaastad, Wisløff, Klungland: "Deletion of mouse Alkbh7 leads to obesity." in: Journal of molecular cell biology, Vol. 5, Issue 3, pp. 194-203, 2013 (PubMed).

Wang, Teng, Di, Rogers, Wu, Kopp, Noguchi: "PPARα and Sirt1 mediate erythropoietin action in increasing metabolic activity and browning of white adipocytes to protect against obesity and metabolic disorders." in: Diabetes, Vol. 62, Issue 12, pp. 4122-31, 2013 (PubMed).

Yekollu, Thomas, OSullivan: "Targeting curcusomes to inflammatory dendritic cells inhibits NF-κB and improves insulin resistance in obese mice." in: Diabetes, Vol. 60, Issue 11, pp. 2928-38, 2011 (PubMed).

Shinmura, Tamaki, Saito, Nakano, Tobe, Bolli: "Cardioprotective effects of short-term caloric restriction are mediated by adiponectin via activation of AMP-activated protein kinase." in: Circulation, Vol. 116, Issue 24, pp. 2809-17, 2007 (PubMed).

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