Uric Acid Colorimetric/Fluorometric Assay Kit

Details zu Produkt Nr. ABIN411711, Anbieter: Anmelden zum Anzeigen
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Reaktivität
Chemical
4
Applikation
Biochemical Assay (BCA)
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Hersteller
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Proben Cell Culture Supernatant, Plasma, Serum, Tissue Samples
Nachweismethode Fluorometric, Colorimetric
Spezifität Uric Acid Assay Kit provides a convenient means for detecting uric acid in biological samples such as serum and urine. Pretreatment of samples are not required. Uric acid level can be measured using fluorometric (at Ex/Em = 535/587 nm ) or colorimetric (at lambda = 570 nm) methods.
Produktmerkmale Uric Acid Assay Kit: Colorimetric & Fluorometric Assay for detecting uric acid in biological samples such as serum and urine within 40 min. Convenient, Simple & Sensitive.
Bestandteile Uric Acid Assay Buffer
Uric Acid Probe (in DMSO, anhydrous)
Uric Acid Enzyme Mix
Uric Acid Standard (2 nmol/μl)
Substanzklasse Chemical
Hintergrund Uric acid in serum is the end product of purine metabolism, and is cleared through the kidney by glomerular filtration. However, human often lacks the necessary enzyme called urate oxidase (Uricase), and therefore abnormal uric acid may be accumulated in blood. Recent evidences show the close association between serum urate level and cardiovascular morbidity and mortality, especially among persons at high cardiovascular risk, including those with hypertension, diabetes and congestion heart failure.
Kommentare

Further details regarding sample type: Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids

Testdauer < 1 h
Protokoll 1. Standard Curve Preparations: For colorimetric assay, add 0, 4, 8, 12, 16, 20 µL into each well individually. Adjust volume to 50 µL/well with Uric Acid Assay Buffer to generate 0, 8, 16, 24, 32, 40 nM/well of Uric Acid Standard. For fluorometric assay, dilute the Uric Acid to 0.2 nM/µL by adding 20 µL into 180 µL of Uric Acid Assay Buffer. Mix well. Add 0, 4, 8, 12, 16, 20 µL into each well individually. Adjust volume to 50 µL/well with Uric Acid Assay Buffer to generate 0, 0.8, 1.6, 2.4, 3.2, 4.0 nM/well of the Uric Acid Standard.
2. Sample Preparations: Prepare test samples in 50 µL/well with Uric Acid Assay Buffer in a 96-well plate. If using serum sample, serum (2-20 µL/assay, normal serum contains approx. 0.3 nM/µL uric acid) can be directly diluted in the Uric Acid Assay Buffer. We suggest using several dilutions to ensure that the readings are within the standard curve range.
3. Reaction Mix Preparation: Mix enough reagents for the number of assays performed: For each well, prepare a total 50 µL Reaction Mix containing: 46 µL Uric Acid Assay Buffer 2 µL Uric Acid Probe 2 µL Uric Acid Enzyme Mix,
4. Mix well. Add 50 µL of the Reaction Mix to each well that contains the uric acid standard and test samples. Incubate the reaction for 30 minutes at 37 °C, protect from light.
5. Measure OD 570nm for colorimetric assay or fluorescence at Ex/Em = 535/590 nm in a microplate reader.
Ergebnisberechnung

Calculation: Correct background by subtracting the reading of no uric acid control from all standard and sample readings (The background reading can be significant and must be subtracted from sample readings). Then apply the sample reading to the standard curve. Uric Acid Concentration C = A/V x 1000 (nM/mL) Where: A is the uric acid amount from the sample well in nmol. V is the sample volume added into the sample well in microliter(s). Uric acid molecular weight is 168.

Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung -20 °C
Haltbarkeit 12 months
Bilder des Herstellers
 image for Uric Acid Colorimetric/Fluorometric Assay Kit (ABIN411711) Uric Acid Colorimetric/Fluorometric Assay Kit
Produkt verwendet in: Hara, Iijima, Elias, Seno, Tojima, Kobayashi, Kephart, Kurabayashi, Kita: "Airway uric acid is a sensor of inhaled protease allergens and initiates type 2 immune responses in respiratory mucosa." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 9, pp. 4032-42, 2014 (PubMed).

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