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KIT ELISA Kit

KIT Reaktivität: Human Colorimetric Sandwich ELISA 31.25-2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN3198414
  • Target Alle KIT ELISA Kits anzeigen
    KIT (Mast/stem Cell Growth Factor Receptor (KIT))
    Reaktivität
    • 9
    • 8
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    31.25-2000 pg/mL
    Untere Nachweisgrenze
    31.25 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.
    Proben
    Cell Culture Supernatant, Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    CD117 / c-Kit, Human
    Sensitivität
    2.16 pg/mL
    Bestandteile
    Plate, Standard, Diluent
    Benötigtes Material
    Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
    Pipettes and pipette tips.
    50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
    Multichannel micropipette reservoir.
    Beakers, flasks, cylinders necessary for preparation of reagents.
    Deionized or distilled water.
    Polypropylene test tubes for dilution.
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  • Probenmenge
    100 μL
    Testdauer
    3 - 4 h
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    Buffer contains: 0.02 % sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Intended for research use only and are not for use in diagnostic or therapeutic procedures.
    Treat all chemicals with caution because they can be potentially hazardous.
    It is recommended that this product is handled only by persons who have been trained in laboratory techniques and in accordance with the principles of good laboratory practice. Wear personal protection equipment such as laboratory coat, safety glasses and gloves.
    Avoid direct contact with skin or eyes. Wash immediately with water in the case of contact with skin or eyes. Avoid contact of skin or mucous membranes with kit reagents or specimens. See material safety data sheet(s) for specific advice.
    Pure water or deionized water must be used for reagent preparation.
    The Stop Solution provided with this kit is an acid solution. Wear personal protection equipment with caution.
    Do not expose kit reagents to strong light during storage and incubation.
    Rubber or disposable latex gloves should be worn while handling kit reagents or specimens.
    Avoid contact of substrate solution with oxidizing agents and metal.
    Avoid splashing or generation of aerosols.
    Use disposable pipette tips and/or pipettes to avoid microbial or cross-contamination of reagents or specimens which may invalidate the test.
    Use clean, dedicated reagent trays for dispensing the conjugate and substrate reagent.
    Exposure to acid inactivates the HRP and antibody conjugate.
    Substrate solution must be warmed to room temperature prior to use.
    Decontaminate and dispose specimens and all potentially contaminated materials as they could contain infectious agents. The preferred method of decontamination is autoclaving for a minimum of 1 hour at 121.5 °C.
    Liquid wastes not containing acid and neutralized waste may be mixed with sodium hypochlorite in volumes such that the final mixture contains 1.0 % sodium hypochlorite. Allow 30 minutes for effective decontamination. Liquid waste containing acid must be neutralized prior to the addition of sodium hypochlorite.
    All reagents including microplate, samples, standards and working solution should be warmed to room temperature before use.
    To obtain accurate results, using adhesive film to seal the plate during incubation is suggested.
    It is recommended that all samples and standards be assayed in duplicate.
    Avoid foaming when mixing or reconstituting solutions containing protein.
    To avoid cross-contamination, use separate reservoirs for each reagent and change pipette tips between each standard, sample and reagent.
    When using an automated plate washer, adding a 30 seconds soak period before washing step and/or rotating the plate between wash steps may improve assay precision.
    When pipetting reagents, maintain a consistent order of addition from well-to-well.
    Keep Substrate solution protected from direct strong light. Substrate Solution should turn to gradations of blue after a proper color development.
    Read absorbance within 30 minutes after adding stop solution.
    Take care not to scratch the inner surface of the microwells.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
    Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
    Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
    Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
    Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
    Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
  • Target Alle KIT ELISA Kits anzeigen
    KIT (Mast/stem Cell Growth Factor Receptor (KIT))
    Andere Bezeichnung
    CD117 / C-Kit (KIT Produkte)
    Synonyme
    C-Kit ELISA Kit, CD117 ELISA Kit, PBT ELISA Kit, SCFR ELISA Kit, Bs ELISA Kit, Fdc ELISA Kit, Gsfsco1 ELISA Kit, Gsfsco5 ELISA Kit, Gsfsow3 ELISA Kit, SCO1 ELISA Kit, SCO5 ELISA Kit, SOW3 ELISA Kit, Ssm ELISA Kit, Tr-kit ELISA Kit, W ELISA Kit, c-KIT ELISA Kit, c-kit ELISA Kit, MGF ELISA Kit, KIT ELISA Kit, C-KIT ELISA Kit, kit ELISA Kit, receptor ELISA Kit, sparse ELISA Kit, Xkl-1 ELISA Kit, kit-a ELISA Kit, kit-b ELISA Kit, kl1-A ELISA Kit, krk1 ELISA Kit, pbt ELISA Kit, scfr ELISA Kit, KIT proto-oncogene receptor tyrosine kinase ELISA Kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a ELISA Kit, KIT proto-oncogene receptor tyrosine kinase S homeolog ELISA Kit, KIT ELISA Kit, Kit ELISA Kit, kita ELISA Kit, kit ELISA Kit, kit.S ELISA Kit
    Hintergrund
    CD117, also known as c-Kit, is a cytokine receptor expressed on the surface of hematopoietic stem cells (HSC) as well as other cell types. CD117 is a receptor tyrosine kinase type III, which binds to stem cell factor.
    Gen-ID
    3815
    NCBI Accession
    NM_000222, NM_001093772
    UniProt
    P10721
    Pathways
    RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
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