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DKK1 ELISA Kit

DKK1 Reaktivität: Ratte AA 23-270 Colorimetric Sandwich ELISA 46.9-3000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN3044704
  • Target Alle DKK1 ELISA Kits anzeigen
    DKK1 (Dickkopf Homolog 1 (DKK1))
    Bindungsspezifität
    AA 23-270
    Reaktivität
    • 6
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    46.9-3000 pg/mL
    Untere Nachweisgrenze
    46.9 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat DKK1
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: S23-H270
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <15pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Immunogen sequence: S23-H270
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plattentyp
    Pre-coated
    Protokoll
    rat DKK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DKK1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: S23-H270) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DKK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat DKK1 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 375.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL rat DKK1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants, serum or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat DKK1 standard solution and each sample is measured in duplicate.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    heparin or EDTA
    Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle DKK1 ELISA Kits anzeigen
    DKK1 (Dickkopf Homolog 1 (DKK1))
    Andere Bezeichnung
    DKK1 (DKK1 Produkte)
    Synonyme
    DKK-1 ELISA Kit, SK ELISA Kit, AI414495 ELISA Kit, SGY ELISA Kit, SGY-1 ELISA Kit, SGY1 ELISA Kit, Soggy ELISA Kit, mSgy ELISA Kit, dkk1 ELISA Kit, dkk ELISA Kit, dkk-1 ELISA Kit, xdkk1 ELISA Kit, Xdkk-1 ELISA Kit, Dickkopf1 ELISA Kit, dickkopf-1 ELISA Kit, mdkk-1 ELISA Kit, dkk1-A ELISA Kit, dickkopf WNT signaling pathway inhibitor 1 ELISA Kit, dickkopf like acrosomal protein 1 ELISA Kit, dickkopf-related protein 1 ELISA Kit, dickkopf-like 1 ELISA Kit, dickkopf WNT signaling pathway inhibitor 1b ELISA Kit, dickkopf protein ELISA Kit, dickkopf WNT signaling pathway inhibitor 1 S homeolog ELISA Kit, DKK1 ELISA Kit, Dkk1 ELISA Kit, DKKL1 ELISA Kit, LOC100228789 ELISA Kit, Dkkl1 ELISA Kit, dkk1b ELISA Kit, dkk1 ELISA Kit, dickkopf ELISA Kit, dkk1.S ELISA Kit
    Hintergrund

    Background: Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

    Synonyms: Dickkopf homolog 1 (Xenopus laevis) (Predicted) ,Dickkopf-like protein 1 ,Protein Dkk1 ,Dkk1 ,DKK1 , Dkk1_predicted , LOC100912050 ,rCG_48380 ,

    Full Gene Name: dickkopf WNT signaling pathway inhibitor 1

    Gen-ID
    293897
    UniProt
    D3Z9J1
    Pathways
    WNT Signalweg, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation
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