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Hsc70 ELISA Kit

HSPA8 Reaktivität: Human, Zebra Finch Colorimetric Sandwich ELISA 2.34 ng/mL - 150 ng/mL Blood, Cell Lysate, Serum, Tissue Samples
Produktnummer ABIN2964823
  • Target Alle Hsc70 (HSPA8) ELISA Kits anzeigen
    Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
    Reaktivität
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Zebra Finch
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    2.34 ng/mL - 150 ng/mL
    Untere Nachweisgrenze
    2.34 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Colorimetric detection of HSC70
    Proben
    Blood, Cell Lysate, Serum, Tissue Samples
    Analytische Methode
    Quantitative
    Sensitivität
    1.54 ng/mL
    Produktmerkmale
    ELISA kit used to quantitate HSC70 concentration in samples.
    Bestandteile
    • Anti-Hsc70 Immunoassay Plate
    • 5X Hsc70 Extraction Reagent
    • Recombinant Hsc70 Standard
    • Standard and Sample Diluent
    • 10X Wash Buffer Concentrate
    • Anti-Hsc70 Biotinylated Antibody Concentrate
    • Anti-Hsc70 Biotinylated Antibody Diluent
    • Streptavidin: HRP Concentrate
    • Streptavidin: HRP Diluent
    • TMB Substrate
    • Stop Solution
    Benötigtes Material
    - Ultra pure water
    - Additional reagents and materials for cell lysate and tissue extract preparation, including protease inhibitors
    - Precision pipettors, with disposable plastic tips
    - Polypropylene or polyethylene tubes to prepare samples − do not use polystyrene, polycarbonate or glass tubes
    - A container to prepare 1X Wash Buffer
    - A wash bottle or an automated 96-well plate washer
    Top Product
    Discover our top product HSPA8 ELISA Kit
  • Testdauer
    0.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare Standard and samples in Standard and Sample Diluent.
    2. Add 100 μL of Standard or sample to appropriate wells.
    3. Cover plate with Plate Sealer and incubate at room temperature (20-25 °C) for 1 hour.
    4. Wash plate four times with 1X Wash Buffer.
    5. Add 100 μL of Biotinylated Antibody Working Solution to each well.
    6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour.
    7. Wash plate four times with 1X Wash Buffer.
    8. Add 100 μL of Streptavidin-HRP Working Solution to each well.
    9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes.
    10. Wash plate four times with 1X Wash Buffer.
    11. Add 100 μL of TMB Substrate to each well.
    12. Develop the plate in the dark at room temperature for 30 minutes.
    13. Stop reaction by adding 100 μL of Stop Solution to each well.
    14. Measure absorbance on a plate reader at 450 nm.
    Testdurchführung
    1. Prepare Standard and samples in Standard and Sample Diluent.
    2. Add 100 μL of Standard or sample to appropriate wells.
    3. Cover plate with Plate Sealer and incubate at room temperature (20-25 °C) for 1 hour.
    4. Wash plate four times with 1X Wash Buffer.
    5. Add 100 μL of Biotinylated Antibody Working Solution to each well.
    6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour.
    7. Wash plate four times with 1X Wash Buffer.
    8. Add 100 μL of Streptavidin-HRP Working Solution to each well.
    9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes.
    10. Wash plate four times with 1X Wash Buffer.
    11. Add 100 μL of TMB Substrate to each well.
    12. Develop the plate in the dark at room temperature for 30 minutes.
    13. Stop reaction by adding 100 μL of Stop Solution to each well.
    14. Measure absorbance on a plate reader at 450 nm.
    Ergebnisberechnung

    Duplicate absorbance values should be within 10% of each other. Care should be taken when interpreting data with differences in absorbance values greater than 10%.

    1. Prepare a standard curve to determine the amount of Hsc70 in an unknown sample. Plot the average absorbance obtained for each standard concentration on the vertical (Y) axis versus the corresponding Hsc70 concentration on the horizontal (X) axis using graph paper or curve-fitting software.

    2. Calculate the Hsc70 concentration in unknown samples using the prepared standard curve. Determine the amount of Hsc70 in each unknown sample by noting the Hsc70 concentration (X axis) that correlates with the absorbance value (Y axis) obtained for the unknown sample.

    3. Multiply the Hsc70 concentration obtained by the dilution factor to determine the amount of Hsc70 in the undiluted sample.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
  • Boĭko, Vetchinin, Sapozhnikov, Kovalenko: "[Alterations in heat shock protein 70 kDa levels in human neutrophils under the heat shock conditions]." in: Bioorganicheskaia khimiia, Vol. 40, Issue 5, pp. 528-40, (2015) (PubMed).

  • Target Alle Hsc70 (HSPA8) ELISA Kits anzeigen
    Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
    Andere Bezeichnung
    HSC70 (HSPA8 Produkte)
    Synonyme
    hsc54 ELISA Kit, hsc70 ELISA Kit, hsc71 ELISA Kit, hsp71 ELISA Kit, hsp73 ELISA Kit, hspa10 ELISA Kit, lap1 ELISA Kit, nip71 ELISA Kit, HSC54 ELISA Kit, HSC70 ELISA Kit, HSC71 ELISA Kit, HSP71 ELISA Kit, HSP73 ELISA Kit, HSPA10 ELISA Kit, LAP1 ELISA Kit, NIP71 ELISA Kit, Hsc70 ELISA Kit, 2410008N15Rik ELISA Kit, Hsc71 ELISA Kit, Hsc73 ELISA Kit, Hsp73 ELISA Kit, Hspa10 ELISA Kit, wu:fb01g06 ELISA Kit, wu:fi48b06 ELISA Kit, heat shock protein family A (Hsp70) member 8 L homeolog ELISA Kit, heat shock protein family A (Hsp70) member 8 ELISA Kit, heat shock 70kDa protein 8 ELISA Kit, heat shock protein 8 ELISA Kit, hspa8.L ELISA Kit, HSPA8 ELISA Kit, Hspa8 ELISA Kit, hspa8 ELISA Kit
    Hintergrund
    HSP70 genes encode abundant heat-inducible 70- kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity. The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half. The structure of this ATP binding domain displays multiple features of nucleotide binding proteins. When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed, HSP 70 is highly related to HSC70 (>90 % sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock.
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