Vasopressin FLISA Kit

Produktnummer ABIN2871261

Produktdetails Vasopressin FLISA Kit

Target: Vasopressin (AVP) (Arginine Vasopressin (AVP))

Reaktivität: Human, Ratte, Maus, Schaf

Nachweismethode: Fluorometric

Methodentyp: Competition ELISA

Applikation: ELISA

Analytische Methode: Quantitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll: The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Antigendetails

Target: Vasopressin (AVP) (Arginine Vasopressin (AVP))

Andere Bezeichnung: Vasopressin (AVP) (AVP Produkte)

Synonyme: ADH FLISA Kit, ARVP FLISA Kit, AVP-NPII FLISA Kit, AVRP FLISA Kit, VP FLISA Kit, 2610201A18Rik FLISA Kit, Akr1a4 FLISA Kit, Vp FLISA Kit, Vsp FLISA Kit, DI FLISA Kit, Vas FLISA Kit, Adh FLISA Kit, Adh1a FLISA Kit, Adh1c FLISA Kit, adh FLISA Kit, arvp FLISA Kit, avrp FLISA Kit, avp-npii FLISA Kit, copeptin FLISA Kit, vasotocin FLISA Kit, arginine vasopressin FLISA Kit, aldo-keto reductase family 1, member A1 (aldehyde reductase) FLISA Kit, alcohol dehydrogenase 1 (class I) FLISA Kit, AVP FLISA Kit, Akr1a1 FLISA Kit, Avp FLISA Kit, Adh1 FLISA Kit, avp FLISA Kit

Pathways: cAMP Metabolic Process