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Vip FLISA Kit

Vip Reaktivität: Human, Ratte, Schwein Fluorometric Competition ELISA
Produktnummer ABIN2871258
  • Target Alle Vip FLISA Kits anzeigen
    Vip (Vasoactive Intestinal Peptide (Vip))
    Reaktivität
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Ratte, Schwein
    Nachweismethode
    Fluorometric
    Methodentyp
    Competition ELISA
    Applikation
    ELISA
    Analytische Methode
    Quantitative
    Featured
    Zu unserem meistverkauften Vip ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
  • Target Alle Vip FLISA Kits anzeigen
    Vip (Vasoactive Intestinal Peptide (Vip))
    Abstract
    Vip Produkte
    Synonyme
    PHM27 FLISA Kit, vip1 FLISA Kit, zgc:171463 FLISA Kit, VIP FLISA Kit, vip-A FLISA Kit, PHI FLISA Kit, vasoactive intestinal peptide FLISA Kit, vasoactive intestinal peptide L homeolog FLISA Kit, vasoactive intestinal polypeptide FLISA Kit, VIP FLISA Kit, Vip FLISA Kit, vip FLISA Kit, vip.L FLISA Kit
    Pathways
    Hormone Activity, cAMP Metabolic Process
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