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Nuclear extracts prepared from C2C12 (undifferentiated) and unstimulated U-937 cells are diluted to 0.625 μg/well and assayed using the TransAM MEF2 Kit. The ratio of the signals from the C2C12 cells over the U-937 cells must be above 3. Lot No. 31603001 was developed for 5.5 minutes. It gave a ratio of 3.6 (Figure 1). The endogenous level of MEF2 expression, and this ratio may vary depending on the cell type tested and the treatment used. TransAM MEF2 Kits are also tested for specificity in detecting MEF2 activity. TransAM MEF2 assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated MEF2 consensus binding site (Figure 2). At 20X excess, the wild-type oligonucleotide prevents MEF2 binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has no effect on MEF2 binding.