RUNX1 ELISA Kit (Runt-Related Transcription Factor 1)

Details for Product RUNX1 ELISA Kit No. ABIN2866252, Anbieter: Anmelden zum Anzeigen
Antigen
  • RUNX1
  • runx1
  • AI462102
  • AML1
  • Cbfa2
  • Pebp2a2
  • Pebpa2b
  • AML1-EVI-1
  • AMLCR1
  • CBFA2
  • EVI-1
  • PEBP2aB
  • runxa
  • Runx-1
  • XAML
  • Xaml1
  • aml
  • aml-1
  • aml1
  • aml1-evi-1
  • amlcr1
  • cbfa2
  • evi-1
  • pebp2ab
  • Aml1
  • uncharacterized LOC473981
  • runt-related transcription factor
  • runt related transcription factor 1
  • runt-related transcription factor 1
  • runt related transcription factor 1 L homeolog
  • LOC473981
  • runt
  • Runx1
  • RUNX1
  • runx1
  • runx1.L
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
8
8
8
Methodentyp
DNA-Binding ELISA
Applikation
ELISA
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Verwendungszweck DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.
Marke TransAM®
Proben Cell Extracts, Tissue Samples
Analytische Methode Quantitative
Nachweismethode Colorimetric
Spezifität TransAM AML-1/Runx1 Kits are tested for sensitivity in detecting AML-1 in nuclear extracts from unstimulated HL-60 or Hela cells.
Produktmerkmale Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied.
Bestandteile One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.
Andere Bezeichnung Aml-1/runx1 (RUNX1 ELISA Kit Abstract)
Applikationshinweise Optimal working dilution should be determined by the investigator.
Kommentare

These extracts are diluted down to 0.625 μg/well and assayed using the TransAM AML-1/Runx1 Kit. The ratio of the signals from the HL-60 over the HeLa must be above 4. Lot No. 30709004 was developed for 6 minutes. It gave a ratio of 38 (Figure 1). The basal level of AML-1 expression, and this ratio, may vary depending on the cell type tested and the stimulation used. TransAM AML-1 Kits are also tested for specificity in detecting AML-1 activation. TransAM AML-1 assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated AML consensus binding site (Figure 2). At 10X excess, the wild-type oligonucleotide prevents AML binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has little effect on AML binding.

Testdauer 5 h
Plattentyp Pre-coated
Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung 4 °C/-20 °C/-80 °C
Informationen zur Lagerung Store the cell extracts at -80°C. All other kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the oligonucleotides, primary antibody, Herring Sperm DNA, DTT and Protease Inhibitor Cocktail that should be kept at -20°C, and the cell extract at -80°C. This product is guaranteed for 6 months from date of receipt.
Haltbarkeit 6 months
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