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VEGFA ELISA Kit

VEGFA Reaktivität: Schwein Colorimetric Sandwich ELISA 40-10.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2748736
  • Target Alle VEGFA ELISA Kits anzeigen
    VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
    Reaktivität
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Schwein
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    40-10.000 pg/mL
    Untere Nachweisgrenze
    40 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Porcine VEGF-A ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: porcine Angiopoietin-1, IL-17F, MIF, Osteoprotegerin, PDGF-BB, RANTES, TGF-alpha, TIMP-1, TIMP-2.
    Sensitivität
    40 pg/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Testdurchführung

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle VEGFA ELISA Kits anzeigen
    VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
    Andere Bezeichnung
    VEGF-A (VEGFA Produkte)
    Synonyme
    MVCD1 ELISA Kit, VEGF ELISA Kit, VPF ELISA Kit, Vegf ELISA Kit, Vegf120 ELISA Kit, Vegf164 ELISA Kit, Vegf188 ELISA Kit, Vpf ELISA Kit, vegf ELISA Kit, vegfa ELISA Kit, wu:fj82c06 ELISA Kit, VEGF-A ELISA Kit, VEGF164 ELISA Kit, eVEGF120 ELISA Kit, eVEGF164 ELISA Kit, vegf-a ELISA Kit, vpf ELISA Kit, vefg ELISA Kit, si:dkey-14d8.3 ELISA Kit, wu:fd42e02 ELISA Kit, vascular endothelial growth factor A ELISA Kit, vascular endothelial growth factor Aa ELISA Kit, vascular endothelial growth factor A L homeolog ELISA Kit, vascular endothelial growth factor Ab ELISA Kit, VEGFA ELISA Kit, Vegfa ELISA Kit, vegfaa ELISA Kit, vegfa.L ELISA Kit, vegfa ELISA Kit, vegfab ELISA Kit
    Hintergrund
    Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF)
    Gen-ID
    397157
    UniProt
    P49151
    Pathways
    RTK Signalweg, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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