TNFRSF12A ELISA Kit

Produktnummer ABIN2748712

Produktdetails TNFRSF12A ELISA Kit

Target: TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.3-80 ng/mL

Untere Nachweisgrenze: 0.3 ng/mL

Applikation: ELISA

Verwendungszweck: Rat TWEAK R (TNFRSF12) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat 4-1BB, Activin A, Adiponectin, B7-1, CD48, CTACK, Decorin, Eotaxin, EphA5, Erythropoietin, FGF-BP, Fit-3 Ligand, Galectin-1, Galectin-3, Gas 1, GFR alpha-1, gp130, HGF, IL-1 R6, IL-1 R alpha, IL-2 R alpha, IL-3, IL-7, IL-17F, IL-22, JAM-A, MIP-1 alpha, Neuropilin-1, Neuropilin-2, Nope, Notch-1, Notch-2, P-Cadherin, Prolactin, RANTES, SCF, TIM-1, TIMP-2, TREM-1

Sensitivität: 0.3 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples4 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Testdurchführung:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu TNFRSF12A

Target: TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))

Andere Bezeichnung: TWEAK R (TNFRSF12A Produkte)

Synonyme: CD266 ELISA Kit, FN14 ELISA Kit, TWEAKR ELISA Kit, AI255180 ELISA Kit, C87282 ELISA Kit, Fn14 ELISA Kit, HPIP ELISA Kit, TWEAK-R ELISA Kit, TweakR ELISA Kit, TNF receptor superfamily member 12A ELISA Kit, tumor necrosis factor receptor superfamily, member 12a ELISA Kit, TNFRSF12A ELISA Kit, Tnfrsf12a ELISA Kit

Hintergrund: Tumor necrosis factor receptor superfamily, member 12a (Type 1 transmembrane protein FN14)

Gen-ID: 302965

UniProt: Q80XX9

Pathways: Apoptose, Regulation of Cell Size