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PRAS40 ELISA Kit

AKT1S1 Reaktivität: Human pThr246 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Produktnummer ABIN2748561
  • Target Alle PRAS40 (AKT1S1) ELISA Kits anzeigen
    PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))
    Bindungsspezifität
    pThr246, total
    Reaktivität
    • 4
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human Phospho-PRAS40 (T246) & Total PRAS40 ELISA Kit. This assay semi-quantitatively measures phophorylated PRAS40 (Thr246) & Total PRAS40 in lysate samples.
    Proben
    Cell Lysate, Tissue Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes human PRAS40 phosphorylated at site Threonine-246 & Total PRAS40
    Produktmerkmale
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product AKT1S1 ELISA Kit
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Testdurchführung

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle PRAS40 (AKT1S1) ELISA Kits anzeigen
    PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))
    Andere Bezeichnung
    PRAS40 (AKT1S1 Produkte)
    Synonyme
    MGC81452 ELISA Kit, lobe ELISA Kit, pras40 ELISA Kit, fb34a04 ELISA Kit, wu:fb34a04 ELISA Kit, Lobe ELISA Kit, PRAS40 ELISA Kit, 1110012J22Rik ELISA Kit, AI227026 ELISA Kit, Lobel ELISA Kit, AKT1 substrate 1 (proline rich) S homeolog ELISA Kit, AKT1 substrate 1 ELISA Kit, AKT1 substrate 1 (proline rich) ELISA Kit, AKT1 substrate 1 (proline-rich) ELISA Kit, akt1s1.S ELISA Kit, AKT1S1 ELISA Kit, akt1s1 ELISA Kit, Akt1s1 ELISA Kit
    Hintergrund
    40 kDa Proline-rich Akt1 Substrate (PRAS40 / AKT1S1 / Lobe) phosphorylated at Threonine-246 & Total PRAS40
    Gen-ID
    84335
    UniProt
    Q96B36
    Pathways
    Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Cell Size, Autophagie, BCR Signaling, Warburg Effekt
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