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CCL3L1 ELISA Kit

CCL3L1 Reaktivität: Schwein Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN2748008
  • Target Alle CCL3L1 ELISA Kits anzeigen
    CCL3L1 (Chemokine (C-C Motif) Ligand 3-Like 1 (CCL3L1))
    Reaktivität
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Schwein
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Porcine (pig) Chemokine (C-C motif) Ligand 3-like 1 (CCL3L1) ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes Porcine Chemokine (C-C motif) Ligand 3-like 1 (CCL3L1)
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product CCL3L1 ELISA Kit
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Testdurchführung

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle CCL3L1 ELISA Kits anzeigen
    CCL3L1 (Chemokine (C-C Motif) Ligand 3-Like 1 (CCL3L1))
    Andere Bezeichnung
    CCL3L1 (CCL3L1 Produkte)
    Synonyme
    464.2 ELISA Kit, D17S1718 ELISA Kit, G0S19-2 ELISA Kit, LD78 ELISA Kit, LD78BETA ELISA Kit, MIP1AP ELISA Kit, SCYA3L ELISA Kit, SCYA3L1 ELISA Kit, C-C motif chemokine ligand 3 like 1 ELISA Kit, chemokine (C-C motif) ligand 3-like 1 ELISA Kit, CCL3L1 ELISA Kit
    Hintergrund
    Chemokine (C-C motif) Ligand 3-like 1 (CCL3L1)
    UniProt
    A1YM34
    Pathways
    Cellular Response to Molecule of Bacterial Origin
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