Activin Receptor Type IC (ACVR1C) ELISA Kit

Details zu Produkt Nr. ABIN2747947, Anbieter: Anmelden zum Anzeigen
Antigen
  • ACVRLK7
  • ALK7
  • Alk-7
  • C230097P10
  • Alk7
  • habrec1
  • activin A receptor type 1C
  • activin A receptor, type IC
  • ACVR1C
  • Acvr1c
Reaktivität
Ratte (Rattus)
Alternativen
Kits mit alternativen Reaktivitäten:
1
Methodentyp
Sandwich ELISA
Applikation
ELISA
Optionen
Hersteller
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Verwendungszweck Custom Rat ALK-7 (ACVR1C/Activin A Receptor) ELISA Kit.
Proben Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode Quantitative
Nachweismethode Colorimetric
Spezifität The antibody pair provided in this kit recognizes Rat ALK-7 (ACVR1C/Activin A Receptor)
Produktmerkmale
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Bestandteile
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Benötigtes Material
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Andere Bezeichnung ALK-7 (ACVR1C ELISA Kit Abstract)
Hintergrund ALK-7 (ACVR1C/Activin A Receptor)
Gen-ID 245921
UniProt P70539
Pathways Negative Regulation of Hormone Secretion, Positive Regulation of Endopeptidase Activity
Probenmenge 100 μL
Plattentyp Pre-coated
Protokoll
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Testdurchführung

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung -20 °C
Informationen zur Lagerung The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit 6 months
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