Vitronectin ELISA Kit
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- Target Alle Vitronectin (VTN) ELISA Kits anzeigen
- Vitronectin (VTN)
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.8-200 ng/mL
- Untere Nachweisgrenze
- 0.8 ng/mL
- Applikation
- ELISA
- Verwendungszweck
- Human Vitronectin ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Proben
- Cell Culture Supernatant, Plasma, Serum
- Analytische Methode
- Quantitative
- Spezifität
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human A2M, ApoA1, ApoC1, ApoC2, ApoC3, ApoE, ApoH, Artemin, BMP-6, Calbindin D, Ck beta 8-1 , CNTF, EMMPRIN, Endostatin, Fibrinogen, FLRG, GITR Ligand, GPBB, Hemoglobin, HTRA2, IL-17E, IL-1R3, Lactoferrin, Mesothelin, PAPP-A, PDECGF, PDGF Ralpha, Pepsinogen II, Periostin, Persephin, PTH, RAP, SAA, Serpin F1, Tie-1, tPA, TSP-2, Ubiquitin+1, Vitronectin, vWF
- Sensitivität
- 0.8 ng/mL
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Applikationshinweise
- Recommended Dilution for serum and plasma samples10,000 fold
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Aufbereitung der Proben
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For example, add 2 μL of serum or plasma into a tube with 198.0 μL 1X Assay Diluent to prepare a 100-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 100-fold diluted sample into a tube with 198 μL 1X Assay Diluent to prepare a final 10,000-fold diluted sample.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
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- Target Alle Vitronectin (VTN) ELISA Kits anzeigen
- Vitronectin (VTN)
- Andere Bezeichnung
- Vitronectin (VTN Produkte)
- Synonyme
- VTN ELISA Kit, DKFZp470F0511 ELISA Kit, LOC100228142 ELISA Kit, vtn ELISA Kit, zgc:112277 ELISA Kit, V75 ELISA Kit, VN ELISA Kit, VNT ELISA Kit, AI256434 ELISA Kit, Vn ELISA Kit, Aa1018 ELISA Kit, vitronectin ELISA Kit, vitronectin a ELISA Kit, vitronectin b ELISA Kit, vitronectin L homeolog ELISA Kit, VTN ELISA Kit, vtn ELISA Kit, vtna ELISA Kit, vtnb ELISA Kit, CpipJ_CPIJ016691 ELISA Kit, vtn.L ELISA Kit, Vtn ELISA Kit
- Hintergrund
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Gene Names: VTN
Protein names: Vitronectin (VN) (S-protein) (Serum-spreading factor) (V75) [Cleaved into: Vitronectin V65 subunit, Vitronectin V10 subunit, Somatomedin-B]
- Gen-ID
- 7448
- UniProt
- P04004
- Pathways
- Autophagie, Smooth Muscle Cell Migration
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