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FLT1 ELISA Kit

FLT1 Reaktivität: Human Colorimetric Sandwich ELISA 1.5-400 ng/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703569
  • Target Alle FLT1 ELISA Kits anzeigen
    FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))
    Reaktivität
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    1.5-400 ng/mL
    Untere Nachweisgrenze
    1.5 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Human VEGFR1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Proben
    Cell Culture Supernatant, Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin A, AgRP, Angiogenin, ANG-1, Angiostatin, Cathepsin S, CD40, Cripto-1, DAN, DKK-1, E-Cadherin, EpCAM, Fas L, Follistatin, Galectin-7, ICAM-2, IL-13 R1, IL-13R2, IL-17B, IL-2R alpha, IL-2 R beta, IL-23, LAP, NrCAM, PAI-1, PDGF-AB, Resistin, SDF-1 beta, sgp130, Shh N, Siglec-5, ST2, TGF-beta 2, Tie-2, TPO, TRAIL R4, TREM-1, VEGF-C.
    Sensitivität
    1.5 ng/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Wood, Cox, Phelps, Lai, Poddar, Talbot, Mu: "Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome." in: Scientific reports, Vol. 6, pp. 19857, (2016) (PubMed).

  • Target Alle FLT1 ELISA Kits anzeigen
    FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))
    Andere Bezeichnung
    VEGFR1 (FLT1 Produkte)
    Synonyme
    FLT ELISA Kit, FLT-1 ELISA Kit, VEGFR-1 ELISA Kit, VEGFR1 ELISA Kit, FLT1 ELISA Kit, flt1b ELISA Kit, sFlt1 ELISA Kit, AI323757 ELISA Kit, Flt-1 ELISA Kit, fms related tyrosine kinase 1 ELISA Kit, FMS-related tyrosine kinase 1 ELISA Kit, vascular endothelial growth factor receptor 1 ELISA Kit, fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) ELISA Kit, FMS-like tyrosine kinase 1 ELISA Kit, vascular endothelial growth factor receptor-1 ELISA Kit, FLT1 ELISA Kit, Flt1 ELISA Kit, CpipJ_CPIJ012087 ELISA Kit, flt1 ELISA Kit, FLT-1 ELISA Kit
    Hintergrund
    Gene Names: FLT1 FLT FRT VEGFR1
    Protein names: Vascular endothelial growth factor receptor 1 (VEGFR-1) (EC 2.7.10.1) (Fms-like tyrosine kinase 1) (FLT-1) (Tyrosine-protein kinase FRT) (Tyrosine-protein kinase receptor FLT) (FLT) (Vascular permeability factor receptor)
    Gen-ID
    2321
    UniProt
    P17948
    Pathways
    RTK Signalweg, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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