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Uromodulin ELISA Kit

UMOD Reaktivität: Human Colorimetric Sandwich ELISA 40-10.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703552
  • Target Alle Uromodulin (UMOD) ELISA Kits anzeigen
    Uromodulin (UMOD)
    Reaktivität
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    40-10.000 pg/mL
    Untere Nachweisgrenze
    40 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Human Uromodulin ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Proben
    Cell Culture Supernatant, Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair detects human Uromodulin. Other species not determined.
    Sensitivität
    40 pg/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
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    Top Product
    Discover our top product UMOD ELISA Kit
  • Applikationshinweise
    Recommended Dilution for serum and plasma samples200 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Cassidy, Slyne, OKelly, Traynor, Conlon, Johnston, Slattery, Ryan, McMorrow: "Urinary biomarkers of chronic allograft nephropathy." in: Proteomics. Clinical applications, Vol. 9, Issue 5-6, pp. 574-85, (2015) (PubMed).

  • Target Alle Uromodulin (UMOD) ELISA Kits anzeigen
    Uromodulin (UMOD)
    Andere Bezeichnung
    Uromodulin (UMOD Produkte)
    Synonyme
    ADMCKD2 ELISA Kit, FJHN ELISA Kit, HNFJ ELISA Kit, HNFJ1 ELISA Kit, MCKD2 ELISA Kit, THGP ELISA Kit, THP ELISA Kit, urehr4 ELISA Kit, uromodulin ELISA Kit, UMOD ELISA Kit, Umod ELISA Kit
    Hintergrund
    Gene Names: UMOD
    Protein names: Uromodulin (Tamm-Horsfall urinary glycoprotein) (THP) [Cleaved into: Uromodulin, secreted form]
    Gen-ID
    7369
    UniProt
    P07911
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