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TRAIL ELISA Kit

TNFSF10 Reaktivität: Maus Colorimetric Sandwich ELISA 2-600 pg/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703528
  • Target Alle TRAIL (TNFSF10) ELISA Kits anzeigen
    TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
    Reaktivität
    • 8
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    2-600 pg/mL
    Untere Nachweisgrenze
    2 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Mouse TRAIL (TNFSF10) ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Proben
    Cell Culture Supernatant, Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair detects mouse TRAIL. Other species not determined.
    Sensitivität
    2 pg/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Ni, Wu, Peterts, Yamamoto, Qing, Nakanishi: "The Critical Role of Proteolytic Relay through Cathepsins B and E in the Phenotypic Change of Microglia/Macrophage." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 36, pp. 12488-501, (2015) (PubMed).

  • Target Alle TRAIL (TNFSF10) ELISA Kits anzeigen
    TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
    Andere Bezeichnung
    TRAIL (TNFSF10 Produkte)
    Synonyme
    APO2L ELISA Kit, Apo-2L ELISA Kit, CD253 ELISA Kit, TL2 ELISA Kit, TRAIL ELISA Kit, A330042I21Rik ELISA Kit, AI448571 ELISA Kit, APO-2L ELISA Kit, Ly81 ELISA Kit, Trail ELISA Kit, tl2 ELISA Kit, apo2l ELISA Kit, cd253 ELISA Kit, trail ELISA Kit, apo-2l ELISA Kit, trail1 ELISA Kit, xtrail1 ELISA Kit, tnfsf10l2 ELISA Kit, zgc:92320 ELISA Kit, TNLG6A ELISA Kit, TNF superfamily member 10 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 10 ELISA Kit, tumor necrosis factor superfamily member 10 ELISA Kit, TNF superfamily member 10 S homeolog ELISA Kit, TNFSF10 ELISA Kit, Tnfsf10 ELISA Kit, tnfsf10.S ELISA Kit, tnfsf10 ELISA Kit
    Hintergrund
    Gene Names: Tnfsf10 Trail
    Protein names: Tumor necrosis factor ligand superfamily member 10 (TNF-related apoptosis-inducing ligand) (Protein TRAIL) (CD antigen CD253)
    Gen-ID
    22035
    UniProt
    P50592
    Pathways
    Apoptose, Positive Regulation of Endopeptidase Activity
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