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TIM3 ELISA Kit
TIM 3
Reaktivität: Human
Colorimetric
Sandwich ELISA
10-2500 pg/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703498
Produktdetails TIM3 ELISA Kit
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Target
Alle TIM3 (TIM 3) ELISA Kits anzeigen
TIM3 (TIM 3)
(Hepatitis A Virus Cellular Receptor 2 (TIM 3))
Reaktivität
Alle Reaktivitäten für TIM3 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
10-2500 pg/mL
Untere Nachweisgrenze
10 pg/mL
Applikation
ELISA
Verwendungszweck
Human TIM-3 (KIM-3) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Proben
Cell Culture Supernatant, Plasma, Serum
Analytische Methode
Quantitative
Spezifität
The antibody pair provided in this kit recognizes Human T Cell Immunoglobulin Mucin-3 (HAVCR2 / KIM-3)
Sensitivität
10 pg/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Applikationshinweise
Recommended Dilution for serum and plasma samples5 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu TIM3
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Target
Alle TIM3 (TIM 3) ELISA Kits anzeigen
TIM3 (TIM 3)
(Hepatitis A Virus Cellular Receptor 2 (TIM 3))
Andere Bezeichnung
TIM-3 (TIM 3 Produkte )
Synonyme
HAVCR2 ELISA Kit, MGC140131 ELISA Kit, HAVcr-2 ELISA Kit, KIM-3 ELISA Kit, TIM3 ELISA Kit, TIMD-3 ELISA Kit, TIMD3 ELISA Kit, Tim-3 ELISA Kit, TIM-3 ELISA Kit, Tim3 ELISA Kit, Timd3 ELISA Kit, tim3 ELISA Kit, hepatitis A virus cellular receptor 2 ELISA Kit, HAVCR2 ELISA Kit, Havcr2 ELISA Kit
Substanzklasse
Virus
Hintergrund
Gene Names: HAVCR2 TIM3 TIMD3 Protein names: Hepatitis A virus cellular receptor 2 (HAVcr-2) (T-cell immunoglobulin and mucin domain-containing protein 3) (TIMD-3) (T-cell immunoglobulin mucin receptor 3) (TIM-3) (T-cell membrane protein 3)
Gen-ID
84868
UniProt
Q8TDQ0
Pathways
Regulation of Lipid Metabolism by PPARalpha , Cancer Immune Checkpoints
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