Syndecan 1 ELISA Kit
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- Target Alle Syndecan 1 (SDC1) ELISA Kits anzeigen
- Syndecan 1 (SDC1)
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 14-4000 pg/mL
- Untere Nachweisgrenze
- 14 pg/mL
- Applikation
- ELISA
- Verwendungszweck
- Human Syndecan-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Proben
- Plasma, Cell Culture Supernatant, Serum
- Analytische Methode
- Quantitative
- Spezifität
- This ELISA antibody pair detects human Syndecan-1. Other species not determined.
- Sensitivität
- 14 pg/mL
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Applikationshinweise
- Recommended Dilution for serum and plasma samples8 fold
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
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- Target Alle Syndecan 1 (SDC1) ELISA Kits anzeigen
- Syndecan 1 (SDC1)
- Andere Bezeichnung
- Syndecan 1 (SDC1 Produkte)
- Synonyme
- CG10497 ELISA Kit, CT27296 ELISA Kit, Dmel\\CG10497 ELISA Kit, Dsyn ELISA Kit, Syd ELISA Kit, Syn ELISA Kit, dSdc ELISA Kit, l(2)10608 ELISA Kit, sdc ELISA Kit, syd ELISA Kit, syn ELISA Kit, Syn1 ELISA Kit, cd138 ELISA Kit, synd1 ELISA Kit, xSyn1 ELISA Kit, syndecan1 ELISA Kit, Syndecan-1 ELISA Kit, XSyndecan-1 ELISA Kit, SDC1 ELISA Kit, CD138 ELISA Kit, SDC ELISA Kit, SYND1 ELISA Kit, syndecan ELISA Kit, AA408134 ELISA Kit, AA409076 ELISA Kit, Sstn ELISA Kit, Synd ELISA Kit, Synd1 ELISA Kit, syn-1 ELISA Kit, HSPG ELISA Kit, SYNDECA ELISA Kit, Syndecan ELISA Kit, Syndecan ELISA Kit, syndecan 1 ELISA Kit, Sdc ELISA Kit, sdc1 ELISA Kit, SDC1 ELISA Kit, Sdc1 ELISA Kit
- Hintergrund
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Gene Names: SDC1 SDC
Protein names: Syndecan-1 (SYND1) (CD antigen CD138)
- Gen-ID
- 6382
- UniProt
- P18827
- Pathways
- Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism
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