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SERPING1 ELISA Kit
SERPING1
Reaktivität: Human
Colorimetric
Sandwich ELISA
2.4-600 ng/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703455
Produktdetails SERPING1 ELISA Kit
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Target
Alle SERPING1 ELISA Kits anzeigen
SERPING1
(Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1))
Reaktivität
Alle Reaktivitäten für SERPING1 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
2.4-600 ng/mL
Untere Nachweisgrenze
2.4 ng/mL
Applikation
ELISA
Verwendungszweck
Human SERPING1 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Proben
Cell Culture Supernatant, Plasma, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes human SERPING1. Other species not yet determined.
Sensitivität
2.4 ng/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples600 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu SERPING1
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Target
Alle SERPING1 ELISA Kits anzeigen
SERPING1
(Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1))
Andere Bezeichnung
Serpin G1 (SERPING1 Produkte )
Synonyme
fb64f02 ELISA Kit, im:7159527 ELISA Kit, si:dkeyp-110g5.2 ELISA Kit, wu:fb60c09 ELISA Kit, wu:fb62a08 ELISA Kit, wu:fb64f02 ELISA Kit, LOC100136072 ELISA Kit, C1-INH ELISA Kit, C1INH ELISA Kit, C1nh ELISA Kit, C1IN ELISA Kit, C1NH ELISA Kit, HAE1 ELISA Kit, HAE2 ELISA Kit, serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 ELISA Kit, C1 inhibitor ELISA Kit, serpin family G member 1 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade G, member 1 ELISA Kit, serping1 ELISA Kit, LOC100136072 ELISA Kit, SERPING1 ELISA Kit, Serping1 ELISA Kit
Hintergrund
Gene Names: SERPING1 C1IN C1NH Protein names: Plasma protease C1 inhibitor (C1 Inh) (C1Inh) (C1 esterase inhibitor) (C1-inhibiting factor) (Serpin G1)
Gen-ID
710
UniProt
P05155
Pathways
Komplementsystem
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