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PEDF ELISA Kit

SERPINF1 Reaktivität: Human, Maus Colorimetric Sandwich ELISA 0.6-160 μg/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703453
  • Target Alle PEDF (SERPINF1) ELISA Kits anzeigen
    PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))
    Reaktivität
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.6-160 μg/mL
    Untere Nachweisgrenze
    0.6 μg/mL
    Applikation
    ELISA
    Verwendungszweck
    Human SERPINF1 (PEDF) ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Proben
    Cell Culture Supernatant, Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA kit detects human Serpin F1. Approximately 40 % cross-reactivity with recombinant mouse Serpin F1 is observed in this antibody pair.
    Sensitivität
    0.6 μg/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle PEDF (SERPINF1) ELISA Kits anzeigen
    PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))
    Andere Bezeichnung
    Serpin F1 (SERPINF1 Produkte)
    Synonyme
    EPC-1 ELISA Kit, OI12 ELISA Kit, OI6 ELISA Kit, PEDF ELISA Kit, MGC82984 ELISA Kit, SERPINF1 ELISA Kit, AI195227 ELISA Kit, Pedf ELISA Kit, Pedfl ELISA Kit, Sdf3 ELISA Kit, Dmrs91 ELISA Kit, zgc:92072 ELISA Kit, serpin family F member 1 ELISA Kit, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 ELISA Kit, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 L homeolog ELISA Kit, serine (or cysteine) peptidase inhibitor, clade F, member 1 ELISA Kit, SERPINF1 ELISA Kit, serpinf1 ELISA Kit, serpinf1.L ELISA Kit, Serpinf1 ELISA Kit
    Hintergrund
    Gene Names: SERPINF1 PEDF PIG35
    Protein names: Pigment epithelium-derived factor (PEDF) (Cell proliferation-inducing gene 35 protein) (EPC-1) (Serpin F1)
    Gen-ID
    5176
    UniProt
    P36955
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