JAG1 ELISA Kit

Produktnummer ABIN2703276

Produktdetails JAG1 ELISA Kit

Target: JAG1 (Jagged 1 (JAG1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.6-400 ng/mL

Untere Nachweisgrenze: 1.6 ng/mL

Applikation: ELISA

Verwendungszweck: Human Jagged 1 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human Jagged 1. Other species not yet determined.

Sensitivität: 1.6 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu JAG1

Target: JAG1 (Jagged 1 (JAG1))

Andere Bezeichnung: Jagged 1 (JAG1 Produkte)

Synonyme: AGS ELISA Kit, AHD ELISA Kit, AWS ELISA Kit, CD339 ELISA Kit, HJ1 ELISA Kit, JAGL1 ELISA Kit, ABE2 ELISA Kit, Gsfabe2 ELISA Kit, Htu ELISA Kit, Ozz ELISA Kit, Ser-1 ELISA Kit, JAG1 ELISA Kit, jag3 ELISA Kit, cb243 ELISA Kit, jag1 ELISA Kit, sb:cb243 ELISA Kit, serrateC ELISA Kit, jagged1 ELISA Kit, serrate ELISA Kit, serrate-1 ELISA Kit, x-serrate-1 ELISA Kit, jagged 1 ELISA Kit, jagged 1b ELISA Kit, jagged 1a ELISA Kit, jagged 1 L homeolog ELISA Kit, JAG1 ELISA Kit, Jag1 ELISA Kit, jag1 ELISA Kit, jag1b ELISA Kit, jag1a ELISA Kit, jag1.L ELISA Kit

Hintergrund: Gene Names: JAG1 JAGL1
Protein names: Protein jagged-1 (Jagged1) (hJ1) (CD antigen CD339)

Gen-ID: 182

UniProt: P78504

Pathways: Notch Signalweg, Stem Cell Maintenance