Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

IL36A/IL1F6 ELISA Kit

IL1F6 Reaktivität: Human Colorimetric Sandwich ELISA 0.8-200 ng/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703145
  • Target Alle IL36A/IL1F6 (IL1F6) ELISA Kits anzeigen
    IL36A/IL1F6 (IL1F6) (Interleukin 1 Family, Member 6 (IL1F6))
    Reaktivität
    • 3
    • 2
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.8-200 ng/mL
    Untere Nachweisgrenze
    0.8 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Human IL-36 alpha (IL-1 F6) ELISA Kit for cell culture supernatants, EDTA and/or Citrate treated plasma, and serum samples. Heparin is not recommended.
    Proben
    Cell Culture Supernatant, Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human IL-1F1, IL-1F2, IL-1F3, IL-1F4, IL-1F5, IL-1F7, IL-1F8, IL-1F9, IL-1F10, IL-1F11.
    Sensitivität
    0.8 ng/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Zu unserem meistverkauften IL1F6 ELISA Kit
    Top Product
    Discover our top product IL1F6 ELISA Kit
  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Aufbereitung der Proben

    Collect plasma using EDTA and/or Citrate as the anticoagulant. Heparin is not recommended.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle IL36A/IL1F6 (IL1F6) ELISA Kits anzeigen
    IL36A/IL1F6 (IL1F6) (Interleukin 1 Family, Member 6 (IL1F6))
    Andere Bezeichnung
    IL-36 alpha (IL1F6 Produkte)
    Synonyme
    Il1f6 ELISA Kit, Fil1 ELISA Kit, IL-1H1 ELISA Kit, IL1RP2 ELISA Kit, Il1f9 ELISA Kit, Il36a ELISA Kit, FIL1 ELISA Kit, FIL1(EPSILON) ELISA Kit, FIL1E ELISA Kit, IL-1F6 ELISA Kit, IL1(EPSILON) ELISA Kit, IL1F6 ELISA Kit, interleukin 36, alpha ELISA Kit, interleukin 1 family, member 6 (epsilon) ELISA Kit, interleukin 1 family, member 6 ELISA Kit, interleukin 36 alpha ELISA Kit, Il36a ELISA Kit, IL1F6 ELISA Kit, IL36A ELISA Kit, Il1f6 ELISA Kit
    Hintergrund
    Gene Names: IL36A FIL1E IL1E IL1F6
    Protein names: Interleukin-36 alpha (FIL1 epsilon) (Interleukin-1 epsilon) (IL-1 epsilon) (Interleukin-1 family member 6) (IL-1F6)
    Gen-ID
    27179
    UniProt
    Q9UHA7
    Pathways
    Cancer Immune Checkpoints
Sie sind hier:
Kundenservice