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LGALS8 ELISA Kit
LGALS8
Reaktivität: Human
Colorimetric
Sandwich ELISA
0.16-40 ng/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703042
Produktdetails LGALS8 ELISA Kit
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Target
Alle LGALS8 ELISA Kits anzeigen
LGALS8
(Lectin, Galactoside-Binding, Soluble, 8 (LGALS8))
Reaktivität
Alle Reaktivitäten für LGALS8 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
0.16-40 ng/mL
Untere Nachweisgrenze
0.16 ng/mL
Applikation
ELISA
Verwendungszweck
Human Galectin-8 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Proben
Cell Culture Supernatant, Plasma, Serum
Analytische Methode
Quantitative
Spezifität
The antibody pair provided in this kit recognizes Human Galectin-8 (LGALS8)
Sensitivität
0.16 ng/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples2 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu LGALS8
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Target
Alle LGALS8 ELISA Kits anzeigen
LGALS8
(Lectin, Galactoside-Binding, Soluble, 8 (LGALS8))
Andere Bezeichnung
Galectin-8 (LGALS8 Produkte )
Synonyme
LGALS8 ELISA Kit, 1200015E08Rik ELISA Kit, AI326142 ELISA Kit, D13Ertd524e ELISA Kit, Lgals-8 ELISA Kit, galectin-8 ELISA Kit, Gal-8 ELISA Kit, PCTA-1 ELISA Kit, PCTA1 ELISA Kit, Po66-CBP ELISA Kit, xgalectin-VIIIa ELISA Kit, galectin 8 ELISA Kit, lectin, galactose binding, soluble 8 ELISA Kit, lectin, galactoside binding soluble 8 S homeolog ELISA Kit, LGALS8 ELISA Kit, Lgals8 ELISA Kit, lgals8.S ELISA Kit
Hintergrund
Gene Names: LGALS8 Protein names: Galectin-8 (Gal-8) (Po66 carbohydrate-binding protein) (Po66-CBP) (Prostate carcinoma tumor antigen 1) (PCTA-1)
Gen-ID
3964
UniProt
O00214
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