FGF7 ELISA Kit

Produktnummer ABIN2703018

Produktdetails FGF7 ELISA Kit

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Reaktivität: Human, Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 6-1.500 pg/mL

Untere Nachweisgrenze: 6 pg/mL

Applikation: ELISA

Verwendungszweck: Mouse FGF-7 (KGF) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects mouse and human FGF-7. Other species not determined.

Sensitivität: 6 pg/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Referenzen für FGF7 ELISA Kit (ABIN2703018)

Ding, Liu, Zeng, Zhu, Liu, Jiang, Huang: "Glycogen synthase kinase‑3β inhibitor reduces LPS‑induced acute lung injury in mice." in: Molecular medicine reports, Vol. 16, Issue 5, pp. 6715-6721, (2018) (PubMed).

Details zu FGF7

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Andere Bezeichnung: FGF-7 (FGF7 Produkte)

Synonyme: HBGF-7 ELISA Kit, KGF ELISA Kit, Kgf ELISA Kit, kgf ELISA Kit, FGF-7 ELISA Kit, fgf7 ELISA Kit, fibroblast growth factor 7 ELISA Kit, fibroblast growth factor 7 L homeolog ELISA Kit, FGF7 ELISA Kit, Fgf7 ELISA Kit, fgf7 ELISA Kit, fgf7.L ELISA Kit

Hintergrund: Gene Names: Fgf7 Fgf-7 Kgf
Protein names: Fibroblast growth factor 7 (FGF-7) (Heparin-binding growth factor 7) (HBGF-7) (Keratinocyte growth factor) (KGF)

Gen-ID: 14178

UniProt: P36363

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung