Fetuin A ELISA Kit
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- Target Alle Fetuin A (AHSG) ELISA Kits anzeigen
- Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))
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Reaktivität
- Maus
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 8-2.000 ng/mL
- Untere Nachweisgrenze
- 8 ng/mL
- Applikation
- ELISA
- Verwendungszweck
- Mouse Fetuin A ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Proben
- Cell Culture Supernatant, Plasma, Serum
- Analytische Methode
- Quantitative
- Spezifität
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse B7-1, BAFF R, BTC, C5a, CCL6, CD48, CD6, Chemerin, Clusterin, CXCL15, Cystatin C, DAN, DLL4, EDAR, Endocan, H60, IL-33, IL-7 Ralpha, Kremen-1, Limitin, Lipocalin-2, LOX-1, Marapsin, MBL-2, Meteorin, Nope, NOV, Osteoactivin, OX40, Ligand, P-Cadherin, Periostin, PlGF-2, Progranulin, Prostasin, Renin 1, Testican 3, TIM-1, TRAIL, Tryptase epsilon
- Sensitivität
- 8 ng/mL
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Applikationshinweise
- Recommended Dilution for serum and plasma samples1,000 fold
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Aufbereitung der Proben
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For example, add 2 μL of serum or plasma into a tube with 38 μL 1X Assay Diluent D to prepare a 20-fold diluted sample. Mix thoroughly and then pipette 5 μL of prepared 20-fold diluted sample into a tube with 245 μL 1X Assay Diluent D to prepare a final 1,000-fold diluted sample.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
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- Target Alle Fetuin A (AHSG) ELISA Kits anzeigen
- Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))
- Andere Bezeichnung
- Fetuin A (AHSG Produkte)
- Synonyme
- ahs ELISA Kit, a2hs ELISA Kit, hsga ELISA Kit, fetua ELISA Kit, wu:fb63g02 ELISA Kit, zgc:103687 ELISA Kit, AHSG ELISA Kit, MGC116429 ELISA Kit, A2HS ELISA Kit, AHS ELISA Kit, FETUA ELISA Kit, HSGA ELISA Kit, Aa2-066 ELISA Kit, pp63 ELISA Kit, alpha-2-HS-glycoprotein ELISA Kit, alpha 2-HS glycoprotein ELISA Kit, alpha-2-HS-glycoprotein 1 ELISA Kit, alpha-2-HS-glycoprotein L homeolog ELISA Kit, Cphamn1_1981 ELISA Kit, AHSG ELISA Kit, ahsg ELISA Kit, ahsg1 ELISA Kit, ahsg.L ELISA Kit, Ahsg ELISA Kit
- Hintergrund
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Gene Names: Ahsg Fetua
Protein names: Alpha-2-HS-glycoprotein (Countertrypin) (Fetuin-A)
- Gen-ID
- 11625
- UniProt
- P29699
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