PTGS2 ELISA Kit

Produktnummer ABIN2702925

Produktdetails PTGS2 ELISA Kit

Target: PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.2-300 ng/mL

Untere Nachweisgrenze: 1.2 ng/mL

Applikation: ELISA

Verwendungszweck: Human COX-2 (PTGS2) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human COX-2. Other species not determined.

Sensitivität: 1.2 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Referenzen für PTGS2 ELISA Kit (ABIN2702925)

Fawzy, Elfayoumi, Mohamed, Fatah, Saadawy: "Cyclooxygenase 2 (rs2745557) Polymorphism and the Susceptibility to Benign Prostate Hyperplasia and Prostate Cancer in Egyptians." in: Biochemical genetics, Vol. 54, Issue 3, pp. 326-36, (2017) (PubMed).

Said, Smith, Sanchez-Carbayo, Theodorescu: "Tumor endothelin-1 enhances metastatic colonization of the lung in mouse xenograft models of bladder cancer." in: The Journal of clinical investigation, Vol. 121, Issue 1, pp. 132-47, (2011) (PubMed).

Details zu PTGS2

Target: PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Andere Bezeichnung: COX-2 (PTGS2 Produkte)

Synonyme: COX-2 ELISA Kit, COX2 ELISA Kit, GRIPGHS ELISA Kit, PGG/HS ELISA Kit, PGHS-2 ELISA Kit, PHS-2 ELISA Kit, hCox-2 ELISA Kit, Cox-2 ELISA Kit, Pghs2 ELISA Kit, TIS10 ELISA Kit, Cox2 ELISA Kit, cox-2 ELISA Kit, CEF147 ELISA Kit, PGHS2 ELISA Kit, PHSII ELISA Kit, cox2 ELISA Kit, gripghs ELISA Kit, pgg/hs ELISA Kit, pghs-2 ELISA Kit, phs-2 ELISA Kit, PTGS2 ELISA Kit, hcox-2 ELISA Kit, fj02a10 ELISA Kit, ptgs2 ELISA Kit, unp1239 ELISA Kit, wu:fj02a10 ELISA Kit, zCOX-2 ELISA Kit, si:dkey-97o5.6 ELISA Kit, prostaglandin-endoperoxide synthase 2 ELISA Kit, prostaglandin G/H synthase 2 ELISA Kit, prostaglandin-endoperoxide synthase 2S homeolog ELISA Kit, prostaglandin-endoperoxide synthase 2a ELISA Kit, prostaglandin-endoperoxide synthase 2b ELISA Kit, PTGS2 ELISA Kit, Ptgs2 ELISA Kit, LOC100136456 ELISA Kit, ptgs2.S ELISA Kit, ptgs2 ELISA Kit, ptgs2a ELISA Kit, ptgs2b ELISA Kit

Hintergrund: Gene Names: PTGS2 COX2
Protein names: Prostaglandin G/H synthase 2 (EC 1.14.99.1) (Cyclooxygenase-2) (COX-2) (PHS II) (Prostaglandin H2 synthase 2) (PGH synthase 2) (PGHS-2) (Prostaglandin-endoperoxide synthase 2)

Gen-ID: 5743

UniProt: P35354

Pathways: Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation