NGFB ELISA Kit

Produktnummer ABIN2702851

Produktdetails NGFB ELISA Kit

Target: NGFB (Nerve Growth Factor beta (NGFB))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 40-10.000 pg/mL

Untere Nachweisgrenze: 40 pg/mL

Applikation: ELISA

Verwendungszweck: Mouse beta-NGF ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: The antibody pair provided in this kit recognizes Mouse Beta-Nerve Growth Factor (bNGF)

Sensitivität: 40 pg/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu NGFB

Target: NGFB (Nerve Growth Factor beta (NGFB))

Andere Bezeichnung: beta-NGF (NGFB Produkte)

Synonyme: NGFB ELISA Kit, ngfb ELISA Kit, ns:zft386 ELISA Kit, xx:zft386 ELISA Kit, zgc:109730 ELISA Kit, hsan5 ELISA Kit, beta-ngf ELISA Kit, Ngfb ELISA Kit, Beta-NGF ELISA Kit, HSAN5 ELISA Kit, beta-NGF ELISA Kit, nerve growth factor ELISA Kit, nerve growth factor b (beta polypeptide) ELISA Kit, beta-Nerve growth factor ELISA Kit, nerve growth factor (beta polypeptide) ELISA Kit, NGF ELISA Kit, ngfb ELISA Kit, FPV072 ELISA Kit, FPV076 ELISA Kit, ngf ELISA Kit, Ngf ELISA Kit

Hintergrund: Gene Names: Ngf Ngfb
Protein names: Beta-nerve growth factor (Beta-NGF)

Gen-ID: 18049

UniProt: P01139

Pathways: NF-kappaB Signalweg, RTK Signalweg, Regulation of Cell Size