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AGT ELISA Kit
AGT
Reaktivität: Human
Colorimetric
Sandwich ELISA
1.22-300 ng/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2702821
Produktdetails AGT ELISA Kit
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Target
Alle AGT ELISA Kits anzeigen
AGT
(Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))
Reaktivität
Alle Reaktivitäten für AGT ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
1.22-300 ng/mL
Untere Nachweisgrenze
1.22 ng/mL
Applikation
ELISA
Verwendungszweck
Human Angiotensinogen (SERPINA8) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Proben
Cell Culture Supernatant, Plasma, Serum
Analytische Methode
Quantitative
Spezifität
The antibody pair provided in this kit recognizes Human Angiotensinogen (AGT/SERPINA8)
Sensitivität
1.22 ng/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples100 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu AGT
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Target
Alle AGT ELISA Kits anzeigen
AGT
(Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))
Andere Bezeichnung
Angiotensinogen (AGT Produkte )
Synonyme
ANHU ELISA Kit, SERPINA8 ELISA Kit, AI265500 ELISA Kit, AngI ELISA Kit, AngII ELISA Kit, Aogen ELISA Kit, Serpina8 ELISA Kit, ANRT ELISA Kit, Ang ELISA Kit, PAT ELISA Kit, wu:fb62f06 ELISA Kit, wu:fj87b02 ELISA Kit, zgc:111892 ELISA Kit, AGT ELISA Kit, angt ELISA Kit, ANGT ELISA Kit, angiotensinogen ELISA Kit, angiotensinogen (serpin peptidase inhibitor, clade A, member 8) ELISA Kit, AGT ELISA Kit, Agt ELISA Kit, agt ELISA Kit
Hintergrund
Gene Names: AGT SERPINA8 Protein names: Angiotensinogen (Serpin A8) [Cleaved into: Angiotensin-1 (Angiotensin 1-10) (Angiotensin I) (Ang I), Angiotensin-2 (Angiotensin 1-8) (Angiotensin II) (Ang II), Angiotensin-3 (Angiotensin 2-8) (Angiotensin III) (Ang III) (Des-Asp[1]-angiotensin II), Angiotensin-4 (Angiotensin 3-8) (Angiotensin IV) (Ang IV), Angiotensin 1-9, Angiotensin 1-7, Angiotensin 1-5, Angiotensin 1-4]
Gen-ID
183
UniProt
P01019
Pathways
JAK-STAT Signalweg , ACE Inhibitor Pathway , EGFR Signaling Pathway , Peptide Hormone Metabolism , Regulation of Systemic Arterial Blood Pressure by Hormones , Regulation of Lipid Metabolism by PPARalpha , Protein targeting to Nucleus , Feeding Behaviour , Monocarboxylic Acid Catabolic Process , Dicarboxylic Acid Transport , Positive Regulation of Response to DNA Damage Stimulus , Regulation of long-term Neuronal Synaptic Plasticity
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