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CD137 ELISA Kit
TNFRSF9
Reaktivität: Maus
Colorimetric
Sandwich ELISA
6-1.500 pg/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2702796
Produktdetails CD137 ELISA Kit
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Target
Alle CD137 (TNFRSF9) ELISA Kits anzeigen
CD137 (TNFRSF9)
(Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))
Reaktivität
Alle Reaktivitäten für CD137 ELISA Kits
Maus
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
6-1.500 pg/mL
Untere Nachweisgrenze
6 pg/mL
Applikation
ELISA
Verwendungszweck
Mouse 4-1BB (TNFRSF9/CD137) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Proben
Cell Culture Supernatant, Plasma, Serum
Analytische Methode
Quantitative
Spezifität
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-13, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Sensitivität
6 pg/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples3-30 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu CD137
(ausblenden)
Target
Alle CD137 (TNFRSF9) ELISA Kits anzeigen
CD137 (TNFRSF9)
(Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))
Andere Bezeichnung
4-1BB (TNFRSF9 Produkte )
Synonyme
TNFRSF9 ELISA Kit, zgc:136557 ELISA Kit, 4-1BB ELISA Kit, CD137 ELISA Kit, CDw137 ELISA Kit, ILA ELISA Kit, A930040I11Rik ELISA Kit, AA408498 ELISA Kit, AI325004 ELISA Kit, Cd137 ELISA Kit, Ly63 ELISA Kit, tumor necrosis factor receptor superfamily member 9 ELISA Kit, TNF receptor superfamily member 9 ELISA Kit, tumor necrosis factor receptor superfamily, member 9a ELISA Kit, tumor necrosis factor receptor superfamily, member 9 ELISA Kit, TNFRSF9 ELISA Kit, tnfrsf9a ELISA Kit, Tnfrsf9 ELISA Kit
Hintergrund
Gene Names: Tnfrsf9 Cd137 Ila Ly63 Protein names: Tumor necrosis factor receptor superfamily member 9 (4-1BB ligand receptor) (T-cell antigen 4-1BB) (CD antigen CD137)
Gen-ID
21942
UniProt
P20334
Pathways
Cancer Immune Checkpoints
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