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IL-21 ELISA Kit

IL21 Reaktivität: Human Colorimetric Cell Culture Cells, Plasma, Serum
Produktnummer ABIN2648760
  • Target Alle IL-21 (IL21) ELISA Kits anzeigen
    IL-21 (IL21) (Interleukin 21 (IL21))
    Reaktivität
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Applikation
    ELISA
    Verwendungszweck
    Human Interleukin 21 (IL-21) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 21 (IL-21) concentrations in cell culture supernates, serum, and plasma.
    Proben
    Serum, Plasma, Cell Culture Cells
    Analytische Methode
    Quantitative
    Sensitivität
    15 ng/ml
    Produktmerkmale
    Human Interleukin 21 (IL-21) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Probenmenge
    100 μL
    Testdauer
    3.5 h
    Plattentyp
    Pre-coated
    Protokoll
    Human Interleukin 21 (IL-21) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-21 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-21 is added to the wells and binds to the combination of capture antibody- IL-21 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-21 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-21 standard dilutions and IL-21 sample concentration determined.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
  • Target Alle IL-21 (IL21) ELISA Kits anzeigen
    IL-21 (IL21) (Interleukin 21 (IL21))
    Andere Bezeichnung
    IL-21 (IL21 Produkte)
    Synonyme
    IL-21 ELISA Kit, Za11 ELISA Kit, interleukin 21 ELISA Kit, IL21 ELISA Kit, Il21 ELISA Kit
    Pathways
    JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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