TGFBR1 ELISA Kit

Produktnummer ABIN2044695

Produktdetails TGFBR1 ELISA Kit

Target: TGFBR1 (Transforming Growth Factor, beta Receptor 1 (TGFBR1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Untere Nachweisgrenze: 0.15 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.069 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Transforming Growth Factor Beta Receptor I (TGFbR1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta Receptor I (TGFbR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta Receptor I (TGFbR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta Receptor I (TGFbR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Transforming Growth Factor Beta Receptor I (TGFbR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu TGFBR1

Target: TGFBR1 (Transforming Growth Factor, beta Receptor 1 (TGFBR1))

Andere Bezeichnung: Transforming Growth Factor beta Receptor I (TGFbR1) (TGFBR1 Produkte)

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Pathways: Growth Factor Binding