MDM2 ELISA Kit

Produktnummer ABIN2043951

Produktdetails MDM2 ELISA Kit

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Untere Nachweisgrenze: 0.15 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.064 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Mdm2 p53 Binding Protein Homolog (MDM2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mdm2 p53 Binding Protein Homolog (MDM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mdm2 p53 Binding Protein Homolog (MDM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mdm2 p53 Binding Protein Homolog (MDM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Mdm2 p53 Binding Protein Homolog (MDM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu MDM2

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Andere Bezeichnung: Mdm2 p53 Binding Protein Homolog (MDM2) (MDM2 Produkte)

Synonyme: ACTFS ELISA Kit, HDMX ELISA Kit, hdm2 ELISA Kit, zgc:109834 ELISA Kit, 1700007J15Rik ELISA Kit, AA415488 ELISA Kit, Mdm-2 ELISA Kit, MDM2 proto-oncogene ELISA Kit, MDM2 proto-oncogene S homeolog ELISA Kit, Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) ELISA Kit, MDM2 oncogene, E3 ubiquitin protein ligase ELISA Kit, transformed mouse 3T3 cell double minute 2 ELISA Kit, MDM2 ELISA Kit, mdm2.S ELISA Kit, Mdm2 ELISA Kit, mdm2 ELISA Kit

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Autophagie, Ubiquitin Proteasome Pathway