Cathepsin B ELISA Kit

Produktnummer ABIN2043091

Produktdetails Cathepsin B ELISA Kit

Target: Cathepsin B (CTSB)

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Untere Nachweisgrenze: 0.15 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.062 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Cathepsin B (CTSB) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin B (CTSB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin B (CTSB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin B (CTSB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cathepsin B (CTSB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Cathepsin B

Target: Cathepsin B (CTSB)

Abstract: CTSB Produkte

Synonyme: APPS ELISA Kit, CPSB ELISA Kit, CB ELISA Kit, apps ELISA Kit, cpsb ELISA Kit, LOC692390 ELISA Kit, CTSB ELISA Kit, cathepsin B ELISA Kit, cathepsin B S homeolog ELISA Kit, CTSB ELISA Kit, Ctsb ELISA Kit, ctsb.S ELISA Kit, LOC692390 ELISA Kit

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades