HSD17B1 ELISA Kit

Produktnummer ABIN2042733

Produktdetails HSD17B1 ELISA Kit

Target: HSD17B1 (Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.61 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu HSD17B1

Target: HSD17B1 (Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1))

Andere Bezeichnung: 17-beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) (HSD17B1 Produkte)

Synonyme: EDH17B2 ELISA Kit, EDHB17 ELISA Kit, HSD17 ELISA Kit, SDR28C1 ELISA Kit, 17HSDB1 ELISA Kit, 17beta-HSD ELISA Kit, E2DH ELISA Kit, Hsd17ba ELISA Kit, 17BHD1 ELISA Kit, zfHSD17B1 ELISA Kit, hydroxysteroid 17-beta dehydrogenase 1 ELISA Kit, hydroxysteroid (17-beta) dehydrogenase 1 ELISA Kit, HSD17B1 ELISA Kit, Hsd17b1 ELISA Kit, hsd17b1 ELISA Kit

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis