FNDC5 ELISA Kit

Produktnummer ABIN2039309

Produktdetails FNDC5 ELISA Kit

Target: FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))

Reaktivität: Human

Nachweismethode: Colorimetric

Detektionsbereich: 3.12-200 ng/mL

Untere Nachweisgrenze: 3.12 ng/mL

Applikation: ELISA

Proben: Serum, Plasma, Tissue Homogenate

Analytische Methode: Quantitative

Kreuzreaktivität (Details): Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.

Sensitivität: 0.78 ng/mL

Bestandteile:

• Assay plate (12 × 8 coated Microwells)
• Standard (freeze dried)
• Biotin-antibody (100 × concentrate)
• HRP-avidin (100 × concentrate)
• Biotin-antibody Diluent
• HRP-avidin Diluent
• Sample Diluent
• Wash Buffer (25 × concentrate)
• TMB Substrate
• Stop Solution
• Adhesive Strip (for 96 wells)
• Instruction manual

Anwendungsinformationen

Applikationshinweise:

• The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
• Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
• Grossly hemolyzed samples are not suitable for use in this assay.
• If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
• Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
• Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
• Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
• Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
• Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.

Kommentare:

Detection wavelength: 450 nm

Information on standard material:
Depending on the antigen to be detected, standards can be either native or recombinant protein. The recombinant proteins are being expressed in CHO cells in most cases. Please inquire for more information. The formulation of auxiliary material in the standard is considered proprietary information, however it does not contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

Information on reagents:
In most cases the stop solution provided is 1 N H2SO4. The formulation of wash solution is proprietary information. None of the components contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

Information on antibodies:
The antibodies provided in different kits vary in regards to clonality and host. Some antibodies are affinity purified, some are Protein A

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.

Handhabung:

• The kit should not be used beyond the expiration date on the kit label.
• Do not mix or substitute reagents with those from other lots or sources.
• If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
• Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
• This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For unopened kit: All the reagents should be kept according to the labels on vials.

Haltbarkeit: 6 months

Referenzen für FNDC5 ELISA Kit (ABIN2039309)

Li, Xu, Wang, Liao, Li, Yang, Gao: "Free androgen index and Irisin in polycystic ovary syndrome." in: Journal of endocrinological investigation, Vol. 39, Issue 5, pp. 549-56, (2017) (PubMed).

Perakakis, Triantafyllou, Fernández-Real, Huh, Park, Seufert, Mantzoros: "Physiology and role of irisin in glucose homeostasis." in: Nature reviews. Endocrinology, Vol. 13, Issue 6, pp. 324-337, (2017) (PubMed).

Dinas, Lahart, Timmons, Svensson, Koutedakis, Flouris, Metsios: "Effects of physical activity on the link between PGC-1a and FNDC5 in muscle, circulating Ιrisin and UCP1 of white adipocytes in humans: A systematic review." in: F1000Research, Vol. 6, pp. 286, (2017) (PubMed).

Li, Yang, Zhou, Fang, Hu, Zhu, Wang, Liu, Li, Liu, Yang, Li: "Elevated circulating levels of irisin and the effect of metformin treatment in women with polycystic ovary syndrome." in: The Journal of clinical endocrinology and metabolism, Vol. 100, Issue 4, pp. 1485-93, (2015) (PubMed).

Palacios-González, Vadillo-Ortega, Polo-Oteyza, Sánchez, Ancira-Moreno, Romero-Hidalgo, Meráz, Antuna-Puente: "Irisin levels before and after physical activity among school-age children with different BMI: a direct relation with leptin." in: Obesity (Silver Spring, Md.), Vol. 23, Issue 4, pp. 729-32, (2015) (PubMed).

Aydin, Aydin, Kobat, Kalayci, Eren, Yilmaz, Kuloglu, Gul, Secen, Alatas, Baydas: "Decreased saliva/serum irisin concentrations in the acute myocardial infarction promising for being a new candidate biomarker for diagnosis of this pathology." in: Peptides, Vol. 56, pp. 141-5, (2015) (PubMed).

Details zu FNDC5

Target: FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))

Andere Bezeichnung: Irisin (FNDC5 Produkte)

Synonyme: FRCP2 ELISA Kit, irisin ELISA Kit, 1500001L03Rik ELISA Kit, AI836596 ELISA Kit, C87088 ELISA Kit, PeP ELISA Kit, Pxp ELISA Kit, fndc5 ELISA Kit, si:dkey-264p5.1 ELISA Kit, zgc:158684 ELISA Kit, fibronectin type III domain containing 5 ELISA Kit, fibronectin type III domain containing 5b ELISA Kit, FNDC5 ELISA Kit, Fndc5 ELISA Kit, fndc5b ELISA Kit

Pathways: Hormone Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation